This research was conducted on the status of growth, biomass, survival and performance of needle-leaved species of Brutia pine (Pinus brutia Ten.) and Aleppo pine (P. halepensis Mill.) with different combinations of organic matter in Koloudeh nursery located in Amol (north of Iran). Seeds in plastic pots were sown as randomized completely design (RCD) with four replications at different treatments of soil including: T1) nursery soil (control), T2) control soil: cattle manure (5:1), T3) control soil:decomposited litter (5:1), T4) control soil: cattle manure: decomposited litter (5:1:1). The results after one year showed that seedlings of T4 obtained better survival rate, shoot height, collar diameter, seedling Vigor Index, shoot dry weight, root dry weight, total dry weight and seedling Quality Index (QI) in both species. A highly positive significant correlation was detected between soil nutrition and characteristics of survival rate, shoot height, collar diameter, shoot dry weight and root dry weight in both species. There was a better response in most of characteristics for P. brutia compared to P. halepensis. It is concluded that higher soil organic matter, due to proper physical and nutritional properties of soil media, increases the seedling quantity and quality.