Microalgaes, with high growing capability and high level of oil yield can be considered as a new source for biodiesel production. In this research, Chlorella vulgariss microalgae was used for biodiesel production. Biodiesel fuel produced was added to diesel fuel with different volume percentages (B2, B5, and B8) and used in the M8.1 diesel engine. The diesel engine was tested at three loads (8.6, 82.9, and %100) and constant engine speed (730 rpm) conditions based on the ECE R-96 standard. Experimental results indicated that using B2, B5, and B8 biodiesel blends does not have a significant effect on brake power and brake Specific fuel consumption. Using B8 at 8.6, 82.9, and %100 loads, the CO emission respectively decreased to 40, 20, and 18%; and the HC emission decreased to 20, 27.2, and 20%, respectively. However, the NOX emission increased to 20, 13.7, and 12.2%, respectively.