Present study has been done to provide empirical evidence for the relationship between earnings quality measure with firm value and the impact of investment opportunity on this relationship.to do so, a comprehensive index based on scaled decile rank of six earnings attributes (e.g. accruals quality, persistence, predictability, value relevance, timeliness, and conservatism) was used as a proxy of earnings quality. Using a sample of 79 firms listed in Tehran Stock Exchange during the period of 1387-1391, we find a positive and significant association between firm valuation with an aggregate earnings quality and investment opportunity. Furthermore, results show that firm’s-Investment opportunities has no significant effect on the relationship between earnings quality and firm value.