مجله طب دامی ایران
سال:1395 | دوره:10 | شماره:1 |تعداد مقالات:14

BACKGROUND: Reports on the pharmacokinetics of marbofloxacin in birds are scarce, even when it is a useful tool in poultry production.OBJECTIVES: Determining marbofloxacin kinetic parameters in plasma and muscle arrangement after intravenous and oral administrations and establishing its withdrawal period.METHODS: Clinical healthy chickens (1.08±0.22 kg) were used as experimental subjects, formed in groups of 5.In Group A (n=45) birds received 2 mg/kg of marbofloxacin intravenously while group B (n=65) was given the same oral dose after fasting for 12 hours. Blood samples in the groups and 5 g of muscle were obtained at different times, in A group up to 24 hours and in B group until 120 hours after application.Once the samples were obtained and treated, marbofloxacin was quantified by HPLC.RESULTS: Marbofloxacin in fasted chickens is almost completely absorbed (F=97%), mean residence time is moderate (6.9 hours), with a high volume of distribution. Orally, mean residence time is less in plasma (4.9 hours) and the withdrawal time was calculated 2.5 days.CONCLUSIONS: The kinetic results are consistent with those presented by other fluoroquinolones in poultry and are in line with short production cycles in chickens.

BACKGROUND: Recognition of pain is challenging in veterinary medicine due to lack of verbal communication and universal pain assessment system. Increase in biochemical parameters have been shown to have direct correlation with level of postoperative pain. OBJECTIVES: The purpose of the present study is to evaluate the serum levels of CPK and investigate any correlation in serum levels of CPK and postoperative pain following ovariohysterectomy in feline.METHODS: Conventional midline ovariohysterectomy was performed in 24 healthy female queens. Serum levels of cortisol, CPK and glucose were measured prior to surgery and at 1 and 3 and 24 hours after the surgery. Also, VAS was used to assess level of post-operative pain. Data was analyzed using repeated measure ANOVA. Spearman’s rank correlation coefficient was used to identify any correlation between level of pain and CPK after the surgery.RESULTS: The score of pain significantly increased at 1 and 3 hours after the surgery in all of the cats (p=0.001). The concentration of cortisol and CPK significantly increased after the surgery in comparison to the values prior to surgery (p<0.001). Also, the concentrations increased significantly at 3 hours after the surgery compared to the values at 1 hour after the surgery (p<0.001). In 24 hours after the surgery results revealed that serum level of cortisol returned to its normal values but CPK was still higher compared to the values prior to surgery. Significant correlation was detected between the level of pain and CPK after surgery (p<0.05). CONCLUSIONS: It is concluded that there is a correlation with VAS score of pain and serum levels of Cortisol and CPK at early hours after the surgery. CPK might be used as an indicator of pain in early hours after the surgery in feline. However, the assessment of pain in cats is not possible based on its values alone.

BACKGROUND: Rhodoccocus equi, Brucella abortus, and Pasteurella multocida are important veterinary bacterial pathogens that in recent years have been resisted to current antibiotics, and this problem threats the livestock industry. To control this resistant microorganisms, use of new antibacterial compounds, such as thiazole derivatives, in veterinary is necessary. OBJECTIVES: In this study, antibacterial effects of thiazole derivatives on Rhodoccocus equi, Brucella abortus, and Pasteurella multocida were evaluated.METHODS: Synthesized thiazole derivatives were prepared in DMSO, then the disk diffusion method was used to measure growth inhibition zone diameter and the broth microdilution method was applied to determine the minimum inhibitory concentration (MIC).RESULTS: Results showed that thiazole derivatives had no significant inhibitory effects on B. abrotus, while they had inhibitory effects on R. equi and P. multocida with inhibition zone 12.7±0.4 -30.1±0.2 mm and MICs 32- 256 mg/ml.CONCLUSIONS: Results of this study indicate that thiazole derivatives have considerable inhibitory effects on R. equi and P. multocida as veterinary bacterial pathogens.