Background: The identification of the factors related to adoption of skin cancer preventive behaviors in adolescents plays a significant role in the appropriate design and implementation of relevant educational approaches. Objectives: The aim of present study was toinvestigate the factors associated with skin cancer preventive behaviors in the male high school students of Isfahan, using the protection motivation theory (PMT). Methods: This descriptive cross-sectional study was conducted on 311 male high school students of Isfahan in 2018. A reliable questionnaire was used for data collection, whose validity and reliability had been confirmed before. The data were analyzed, using the SPSS software (version 20), Pearson’ s correlation coefficient, multiple linear regression, one-way ANOVA, independent t test, and Spearman’ s correlation coefficient. Results: Pearson’ s correlation coefficient indicated that the score of skin cancer preventive behaviors was inversely associated with response cost score (P < 0. 001), while it was directly related to perceived susceptibility, perceived severity, self-efficacy, response efficacy, fear, and protection motivation (P < 0. 001). The multiple linear regression model showed that among the constructs of the PMT, the scores for self-efficacy, response cost, and perceived susceptibility were significant predictors of skin cancer preventive behaviors, respectively, in order of importance. Conclusions: This study showed that the PMT constructs were associated with the skin cancer preventive behaviors, which could be promoted by designing and implementing educational studies based on the constructs.