Context: Infertility is a global health problem characterized by inability of couple to achieve pregnancy after one year of regular unprotected sexual intercourse; or after 6 months if the woman is older than 35 years of age. Aims: To examine the effect of determinants on the Utilization of Assisted Reproductive Technology (ART) among women Setting and Design: This is a cross sectional survey design conducted on women at the Obstetrics, Gynaecology and Fertility clinics in university of Benin Teaching Hospital, Benin-City, Nigeria, 2020. Material and Methods: This study was conducted among 348 women, selected through stratified probability sampling method, and a self-structured questionnaire was designed, administered and same retrieved. Statistical Analysis Used: Data collected were analyzed using descriptive statistics, means, standard deviation (SD) and Pearson Correlation Coefficient at 0. 05 significant levels. Results: The mean (SD) of the determinants: religion, culture, cost, ethics, and husband perception were as follows: 2. 47 ± 1. 28, 2. 34 ± 1. 20, 3. 37 ± 1. 25, 2. 35 ± 1. 09, 2. 33 ± 1. 13 respectively. The Pearson Correlation Coefficient, shows significant relationship (P=<0. 001, P=0. 023) between cost, husband perception and the women decision, while there are no statistical relationship (P=0. 591, P=0. 892, P=0. 934) between religion, culture, ethics and the decision for ART among women. Conclusion: The cost of ART is a major factor, therefore, there is need for international efforts to facilitate globalization of ART services with special attention to manpower development, subsidization of the running cost and establishment a global partnership as obtained in the Sustainable Development Goals (SDGs).