In order to evaluate the effect of crops rotation on agronomical and non agronomical characters of cotton in KarkandehStation in Golestan Proviences.The first and second years planted cotton and stems were cut to pieces and ploughed in soil.In the third and fourth years 15 treatments includes: soybean, barley, onion, ploughing, ploughing+100kg nitrogen, tomato, wheat, lettuce, broad bean, green bean, green pea, sesame, sorghum, colsa based on randomized complete block design withfour replications were planted. The yield, boll weight, percentage of earliness, sympodial length, monopodial number, monopodiallength, disease indexes of wilt and Verticillium wilt percent were measured. Population of Verticillium dahliae, in driedsoil were assessed before and after treatments by using semi selective Alcohol agar medium. The results were compared usingDuncan’s multiple range test. The results showed that the highest yield of cotton in cotton+urea+cotton, and plowing+plowing+and cotton+, sorghom+cotton, and lowest yield in succession, canola+forage barly+cotton and Cotton+Cotton+garden cress, widest monopodial length forage barly+cotton+cotton and the highest of sympodial length, cotton+sorghom+cotton, that the highest of monopodial number, barely+spinach+cotton and, the highest percentage of earliness barely+spinach+cotton were measured. Percent increase in the population of ofVerticillium dahliae in cotton+Cotton+Cotton+Cotton+Cotton sequence from first to fifth respectively 52/5, 25, 5/13, 5/6 and 0, respectively.The results showed that, populationofVerticillium dahliae, were incresed after cotton in first year (35%- 70%), in second year (14%- 36%), third year (12%-15%), fourth year (4% - 9%) and fifth year without increase. Population ofVerticillium dahliae, were increased after wheat (66.7%), barley (68%), colsa (33.3%0, forage barly (48.5%- 54.5%), lettuce (25%- 39%), onion (52%), soybean (36%), ploughing (36%), ploughing+100kg nitrogen (50%), green bean (12.5%) and sorghum (62.5%). population ofVerticillium dahliae, were decresedafter tomato (6.6%), and sesame (26.6%).