Background and Objective: Workers in many industries, such as oil, gas, petrochemicals, cement, steel, glass, and textile, as well as people at home and offices, are exposed to various noises with different frequencies, causing hearing and non-hearing impairments. The present study aimed to assess the effect of noise at different frequencies on physiological responses and cognitive functions of the personnel working in the oil drilling industry and urban gas pressure reducing stations. Materials and Methods: This descriptive-analytical study was conducted on 50 employees of oil drilling rigs (low-frequency noise) and urban gas pressure reducing stations (high-frequency noise) in 2019. The examined physiological parameters included heart rate, systolic and diastolic blood pressure, as well as tympanic body temperature. The participants' cognitive functions were assessed using an advanced reaction timer, continuous performance, and working memory tests before, during and, after the activities. Results: Based on the obtained results, exposure to noise at high and low frequencies increased physiological responses, including heart rate, systolic and diastolic blood pressure, and tympanic temperature, and this increase was statistically significant (P<0. 001). Moreover, exposure to noise has a negative effect on cognitive functions (such as working memory, sustained attention, and advanced reaction timer). Conclusion: As evidenced by the results of the present study, low-frequency noise had a greater effect on physiological responses and cognitive function, as compared to high-frequency noise. Nonetheless, further research is suggested to be conducted in the industry in this area in the future.