This research was carried out to product the neomale monosex population of molly fish (Poecilia latipinna) with consuming oral type of 17-a methyl testosterone hormone in two experimental groups of brood and fry molly fish in eight treatments (group one: 0, 100, 200, 300 mg hormone / kg food and group two: 0, 300, 400, 500 mg hormone / kg food) for 60 days. At the end of the experiment period, histological and morphological studies along with calculating amount of diurnal growth (ADG), special growth rate (SGR), mortality rate, neomale production percent, sterilization percent and mixed sex percent was carried out to determine the best dose in commercial production. The results showed that second treatments of each exam (B=200 mg hormone and F = 400 mg hormone / kg food) had the best ADG and SGR, the minimum mortality, maximum level of masculinization in morphological view and 90% of neomale populations in histological view. These treatments produced maximum neomale monosex populations and were economic and useful, while the third treatments of each exam despite of having 100% masculinization rate based on morphological studies, had high mortality and 60% masculinization rate based on histological studies.