Twelve species of the genus Aphelenchoides were collected and identified based on morphological and morphometric characters from different regions in North, South and Western Iran. Among collected species i. e. A. centralis, A. cibolensis, A. cyrtus, A. hamatus, A. helicus, A. huntensis, A. limberi, A. obtusicaudatus, A. sprophilus, A. sacchari, A. spicomucronatus and A. tuzeti, three species namely A. cibolensis, A. hamatus and A. saprophilus arenew records from Iran. Description, measurements, line drawings andmicroscopic photographs for Iranian population of these species are provided. Aphelenchoides helicus that was previously reported without description, isdescribed and illustrated as well.