مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Issue Information

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Issue Info: 
  • Year: 

    2024
  • Volume: 

    17
  • Issue: 

    5
  • Pages: 

    1-9
Measures: 
  • Citations: 

    0
  • Views: 

    18
  • Downloads: 

    16
Abstract: 

Background: The rapid spread of multidrug-resistant Gram-negative bacteria, particularly the increase in carbapenem-resistant strains, has led to a critical level of resistance in clinical settings. Colistin is considered to be the last line of defense against these Gram-negative bacteria. Objectives: This study aims to investigate the genetic characteristics and antimicrobial resistance (AMR) patterns of four mcr-1 (mobile colistin resistance) Escherichia coli isolates from various sources in Hainan Province, tropical China, and to determine their genetic relationships with both domestic and global strains. Methods: Samples were collected from swimming pools, marine beaches, and beach personnel in Haikou and Sanya, Hainan Province, China. Escherichia coli isolates were obtained, multilocus sequence typing was performed, and the mcr family resistance genes were amplified by polymerase chain reaction. The mobile sequences, AMR genes, phenotypes, and virulence genotypes of the mcr-1 strains were analyzed. Phylogenetic analyses were conducted to investigate the relationships between the mcr-1 E. coli strains and those in the NCBI database. Results: In the current study, the mcr-1 resistance gene was detected in 4 strains, accounting for 2. 94% (4/136) of the total. The 4 strains of E. coli were isolated from a freshwater swimming pool, a pool wall, seawater, and a human body. Among the four strains, the minimum inhibitory concentration of polymyxin was 8 mg/L, except for one strain, which showed low-level resistance at 4 mg/L. All four strains showed complete resistance to ampicillin, three were resistant to compound sulfamethoxazole and chloramphenicol, and all strains were sensitive to other common antibiotics. The virulence genes cfaA, cfaB, cfaC, and cfaD/cfaE were detected in all E. coli strains. The insertable sequence IS3 was the most widely distributed type. Three isolates were identified as ST987, whereas the isolate from marine sources was identified as ST24. Comparison of the core genes of the strains indicated that the four strains were closely related to the Chrysomya sp. from Northern Thailand. Conclusions: The four mcr-1 genes can mediate low to medium levels of colistin resistance, facilitating their dissemination within the population and the environment. It is crucial to trace the source of multidrug-resistant bacteria in recreational water, which is in direct contact with humans, and to reduce the risks to human health through effective supervision.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2024
  • Volume: 

    17
  • Issue: 

    5
  • Pages: 

    1-8
Measures: 
  • Citations: 

    0
  • Views: 

    19
  • Downloads: 

    12
Abstract: 

Background: The factors responsible for the progression of COVID-19 from a mild illness to a severe and often lethal condition, characterized by respiratory failure and multiple organ involvement, remain unclear. The identification of biomarkers capable of predicting disease progression is of the highest importance. Objectives: This study sought to assess laboratory measurements of interferon-gamma inducible protein-10 (IP-10), macrophage inflammatory protein 1-alpha (MIP1α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) to achieve this objective. Methods: We measured IP-10 and MIP1α by qRT-PCR and IL-6 and IL-1β using an enzyme-linked immunosorbent assay in 120 serum samples. We analyzed differences between patients with moderate, severe, and recovered COVID-19. Results: The number of positive cases for biomarkers IP-10, MIP1α, IL-6, and IL-1β were significantly different between groups. The expression levels of IP-10 and MIP1α were significantly higher in patients with severe COVID-19 compared to those who had recovered. A strong positive association was observed between IP-10 and MIP1α in severe infection cases. Additionally, these biomarkers were relatively independent predictors of disease severity. Conclusions: The results suggest that IP-10, MIP1α, IL-6, and IL-1β are promising research candidates for understanding the severity of COVID-19 and for investigating possible pathophysiological mechanisms of the disease.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic ResourcesDownload 12 مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic ResourcesCitation 0 مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic ResourcesRefrence 0
Issue Info: 
  • Year: 

    2024
  • Volume: 

    17
  • Issue: 

    5
  • Pages: 

    1-7
Measures: 
  • Citations: 

    0
  • Views: 

    14
  • Downloads: 

    38
Abstract: 

Background: Members of the phylum Verrucomicrobia, especially Akkermensia muciniphila, have been identified as beneficial gut bacteria. Changes in the gut microbiota are associated with the occurrence of metabolic diseases, such as diabetes, obesity, and irritable bowel syndrome,however, there is conflicting information about their role and changes in colorectal cancer (CRC). Objectives: In the present study, the prevalence of this bacterial phylum in the intestines of individuals with colorectal lesions and healthy controls was investigated. Methods: Mucus samples were obtained from the colons of 69 individuals with colorectal lesions, including CRC and precancerous gut lesions (PCL), and 68 individuals without lesions during colonoscopy. Bacterial DNA was extracted from the mucus samples. A conventional polymerase chain reaction (PCR) assay was performed to detect Verrucomicrobia using a specific 16S rRNA primer. Results: The prevalence of Verrucomicrobia was greater in healthy individuals (75. 4%) than in those with either cancer or PCL (59. 7%) (P = 0. 038). However, there was no significant difference in the presence of these bacteria between the CRC and PCL patient groups. A reverse correlation was observed between the presence of Verrucomicrobia and both chronic constipation (P = 0. 001) and anemia (P = 0. 048). Conclusions: A decreased frequency of Verrucomicrobia in humans may serve as the basis for the initiation of intestinal changes, resulting in the development of colorectal precancerous and cancerous lesions.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Author(s): 

Huang Chienhsiu

Issue Info: 
  • Year: 

    2024
  • Volume: 

    17
  • Issue: 

    5
  • Pages: 

    1-6
Measures: 
  • Citations: 

    0
  • Views: 

    18
  • Downloads: 

    4
Abstract: 

Background: The potentially developing human pathogen Alcaligenes faecalis is a Gram-negative, nonfermenting bacterium. Treatment of A. faecalis infections is frequently difficult due to increased resistance to many antibiotics, such as β-lactam antibiotics, aminoglycosides, and quinolones. In the literature, A. faecalis pneumonia has never been documented in a case series study. Here, we provide a case series study of patients with A. faecalis pneumonia. Objectives: The aim of this study was to elucidate the clinical characteristics, management strategies, and outcomes of A. faecalis pneumonia patients. Methods: Patients with A. faecalis pneumonia between January 2014 and December 2019 were included in a retrospective investigation. We examined the clinical outcomes of patients with A. faecalis pneumonia, together with the risk factors for pneumonia, prior intravenous antibiotic use within 90 days, respiratory secretion culture results, antibiotic sensitivity test results, and other variables. Results: Observations revealed eight patients with A. faecalis pneumonia, including six males and two females. The mean age of patients was 70 years. These eight patients shared risk factors for pneumonia. Six patients were discharged, and two patients died as a result of A. faecalis pneumonia. Two patients with pneumonia caused by extensively drug-resistant A. faecalis were identified. Conclusions: Rare cases of A. faecalis pneumonia have been documented in the literature. Recently, A. faecalis has developed extensive drug resistance. To treat pneumonia caused by A. faecalis with substantial antibiotic resistance, either polymyxin B or tigecycline is needed. The clinical outcome is typically satisfactory when A. faecalis pneumonia patients receive appropriate antibiotic therapy, but cases of fatal pneumonia have been documented in the literature.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Author(s): 

Cizmeci Zeynep

Issue Info: 
  • Year: 

    2024
  • Volume: 

    17
  • Issue: 

    5
  • Pages: 

    1-9
Measures: 
  • Citations: 

    0
  • Views: 

    20
  • Downloads: 

    42
Abstract: 

Background: Carbapenem resistance is one of the major global public health threats caused by the overuse or misuse of carbapenems, which are often the last resort in treating multidrug-resistant infections. Today, timely detection of carbapenemase-producing organisms is critical for infection control. Therefore, rapid and accurate detection of carbapenemases using a cost-effective method with high sensitivity and specificity is essential to guide appropriate treatment and prevent further spread. Objectives: This study aimed to evaluate the sensitivity of the newly developed rapid carbapenemase detection method (rCDM) with isolates whose resistance genes were determined by molecular methods and to compare it with the modified carbapenem inactivation method (mCIM), whose diagnostic performance characteristics are well-defined. Methods: A total of 130 Klebsiella pneumoniae isolates (65 carbapenem-susceptible, 65 carbapenem-resistant) from various clinical specimens were included in the study. Identification and antibiotic susceptibility testing of the isolates was performed using the BD Phoenix™ automated system. Phenotypic carbapenemase detection methods, mCIM and rCDM, were studied for all carbapenem-sensitive and carbapenem-resistant isolates with known resistance genes. Results: Among carbapenem-resistant K. pneumoniae isolates, bla OXA-48 was the most frequently detected resistance gene (78%). Using PCR results as the reference method, the sensitivity of rCDM was 100%. When rCDM and mCIM results were compared, both phenotypic methods were 100% compatible, with no statistically significant difference observed. Conclusions: Rapid carbapenemase detection method is a rapid and reliable phenotypic carbapenemase detection method with high sensitivity. Its ease of use and interpretation make it suitable for use in laboratories with limited resources. Additionally, its excellent performance in detecting common carbapenemase types and high consistency with mCIM support its utility in various laboratory settings without requiring additional materials or technical infrastructure.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2024
  • Volume: 

    17
  • Issue: 

    5
  • Pages: 

    1-9
Measures: 
  • Citations: 

    0
  • Views: 

    16
  • Downloads: 

    26
Abstract: 

Background: The incidence of invasive aspergillosis and the administration of voriconazole have risen among immunocompromised patients. Objectives: This study aimed to evaluate serum voriconazole concentration and its corresponding influential factors in pediatric patients with hematologic disorders. Methods: A total of 132 blood samples were collected from 44 pediatric patients with hematologic disorders infected with invasive aspergillosis and treated with voriconazole. Among these patients, 20. 5% were classified as having proven invasive aspergillosis, 77. 2% as probable, and 2. 3% as possible. Voriconazole serum levels were evaluated using HPLC on the 3rd, 5th, and 7th days of treatment. Genotyping of the CYP2C19 alleles (*2, *3, and *17) was performed, and demographic and clinical data were gathered from records between 2018 to 2020. Results: The voriconazole concentration in 70. 5% of patients and 77. 3% of treatment cases (complete or partial) ranged from 1 to 5. 5 µg/mL. Adverse events were observed in 4. 5% of the patients. Genotyping of CYP2C19 genes revealed CYP2C19*1*1 (5. 4%), CYP2C19*1*17 (16. 2%), CYP2C19*1*2 (51. 4%), and CYP2C19*2*17 (27%). Multivariate analysis using linear regression demonstrated that serum voriconazole concentration increased by 0. 037 µg/mL per year of age and by 0. 06 µg/mL for each unit increase in C-reactive protein (on the 3rd day of voriconazole therapy). Additionally, an increase in alanine aminotransferase level by 1 unit decreased the mean voriconazole concentration by 0. 03 µg/mL. Of these patients, 65. 9% were completely treated, 11. 4% were partially treated, and 22. 7% died. Conclusions: Serum voriconazole concentrations varied among pediatric hematologic patients receiving standard doses, with age, C-reactive protein, and alanine aminotransferase levels affecting the concentration of voriconazole in the sera of pediatric patients.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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