Background: Fear, anxiety and pain of delivery have the important role in the passing way of the delivery process. Some clear-sighted people believ that threshold of pain differs among ethnic groups. Therefore, this study was conducted to compare primipara’s women perception of pain, fear and anxiety of vaginal delivery among Persian, Kurdish and Turkish women.Methods: In this descriptive-analytical study, 450 primipara’s women selected from general hospitals of Shiraz, Tabriz and Kermanshah city using multistep random-sampling method were assessed. Data collection was done using questionnaire and visual analogue scale of pain (VAS). Data were analyzed using descriptive and analytic statistics (mann-withneyu, Kruskal-wallis) and SPSS.16 software. In this study statistically P<0.05 was significant.Results: The mean of pain severity in primipara’s Persian, Kurdish and Turkish women were (8.24±1.21), (7.01 ±1.98) and (7.82 ±0.79) respectively. Kruskal-wallis test showed that severity of pain was significant amonge three groups. The mean of fear and anxiety in primipara’s Persian, Kurdish and Turkish women were (55.64±14.66), (42.11 ±8.81) and (40.11 ±14.56) respectively and was significantly different amonge three groups.Significant relationship was found between maternal education with childbirth pain perception among Turkish women (P<0.001).Significant relationship was found between maternal jobs with childbirth pain perception among three groups of women.Conclusion: perception of pain severity, fear and anxiety of vaginal delivery was significantly different amonge Persian, Kurdish and Turkish women. It seems that different issues such as cultural, social, mental, environmental and psychological factors affect woman’s perception of labor pain.