Background: The present work deals with examine the awareness and attitudes of specialists of oral diseases, pathology and maxillofacial surgery in 2022 in oral exfoliative cytology.Methods: This study was an analytical and cross-sectional research. The statistical population of the present study was Iranian oral diseases specialists, pathologists and maxillofacial surgeons. A researcher-made questionnaire was given to the specialists by senior student and then they were asked to complete it . The questionnaire was given to people by the final year student. The obtained results were analyzed using the T-test, Chi-Square and SPSS-18 Software. The significance level in data analysis was considered by P <0.05 level.Results: A total of 210 questionnaires were distributed in the study, of which 192 were examined (Response Rate = 91.4%). The study revealed that only 18 participants used exfoliative cytology technique. Moreover, 62% of people had a positive attitude towards the application and performance of cytology. There was a significant relationship between the positive attitude, field of study, graduation year and age. The mean scores of awareness in men and women were 32.38 ± 4.21 and 34.42 ± 3.89 , respectively. The participants had a high level of awareness and attitude towards this technique.Additionally, the mean score of awareness in oral specialists, surgeons and pathologists was 33.12±4.23, 33.65±5.12 and 33.45±5.34, respectively. The study revealed no significant relationship between awareness score, field of study and gender. Nonetheless, there was a significant relationship between graduation year, age and awareness score.Conclusion: The study revealed that only 9.4 percent of participants used exfoliative cytology technique. However, they had a high level of awareness and attitude towards this technique.Keywords: Awareness, Attitude, Exfoliative Cytology, Dentistry