In this research, the effect of various amounts of aluminum on microstructure and matrix hardness of ductile cast irons produced by in-mold casting process is investigated. For this purpose, spherical graphite cast iron containing 3. 7, 6. 4 and 7. 5 wt. % aluminum, respectively, were prepared in Y-block form via in-mold process. After casting, samples were prepared for microstructural studies, using conventional methods, and then were examined by optical and scanning electron microscopy. Chemical composition of samples was determined using optical emission spectrometry (OES), X-ray fluorescence (XRF), and carbon-sulfur analyzer (LECO) method. X-ray diffraction test (XRD) was used for phase identification. The results indicate changing of the precipitated pearlite morphology as the aluminum content increases. Also pearlite and ferrite hardness increases, 178 and 164 Vickers, respectively, as a result of increasing aluminum content from 3. 7 to 7. 5 wt. %. Increasing the number of nodular graphites from 422 to 668 per square millimeter, reducing the graphite diameter from 13. 1 to 8. 8 micrometers, and changing the percentage of spherical graphite from 60 to 48 percent, with increasing aluminum content, are other noteworthy results.