The 20m shuttle run test (20mSRT) is applied for assessing cardiorespiratory fitness, and is included in manyfitness test batteries, such as Eurofit, Alpha-fit, FitnessGram, etc.The aim of this study was to validate software, specially designed by us (BeepShuttle Junior), which administersthe 20mSRT, calculates the maximal oxygen uptake (VO2max) in children and adolescents from 6 to 17 years ofage, and classifies them in accordance with comprehensive gender- and age-specific international norms. Thissoftware supports the administration of the 20mSRT with an appropriate animation and audio signals. The mostrecent VO2max reference standards were combined in order to assess cardiorespiratory fitness. The results canbe exported in an Excel compatible CSV file for additional analyses.A total of 63 children (31 girls and 32 boys) from London, aged from 6 to 9, were tested with the BeepShuttleJunior software, which calculated VO2max and percentile scores for each child.The boys performed significantly better than girls in cardiorespiratory fitness (47.7 ± 3.1 vs 45.9 ± 2.2ml/kg/min respectively, p < 0.05), but the mean percentile scores for boys and girls were similar (53.2 ± 23.6and 53.9 ± 22.1 respectively).This software computes the percentile score for the exact age (years, months) and the exact VO2max of eachparticipant by linear interpolation. The individual results and assessments completely matched the interpolatednormative data tables.