Objective: The aim of this study was to investigate the effect of phosphoric acid re-etching of an enamel surface treated via a one-bottle adhesive system on shear bond strength between resin composite and the enamel surface in different stages of adhesive application. Materials and Methods: Extracted intact premolars (n=84) were divided into seven groups (n=12). In the control group 1, the adhesive i-Bond was used according to the manufacturer's instructions, with nocontamination. In groups 2 to 4, the conditioned and saliva, contaminated enamel was blot dried only, rinsed, and blot dried, rinsed blot dried and re-etched, respectively. In groups 5, 6and 7 cured adhesive was contaminated with saliva and then rinsed and blot-dried, blot dried only and rinsed, blot-dried and re-etched respectively. In groups 3, 4, 6 and 7 the adhesive was reapplied. Afterward, Z100 compos-ite cylinders were bonded to the enamel surfaces. The samples were thermocycled (5°C and 55°C, 30 s, dwelling time: 10 s, 500 cycles). Finally, the samples were sheared using Dartec testing machine and shear bond strength data were subjected to one-way ANOVA analysis and Tukey's HSD test. Results: There were statistically significant differences among groups 1 and 5-7. The samples in groups 1 and 4 demonstrated high-er bond strengths than those in the other groups. Conclusion: Using phosphoric acid etching may be effective, only where contamination occurs prior to curing of the adhesive. Af-ter curing of the adhesive, none of the methods in this study would be preferred.