Background: Stillbirth is an important economic, cultural, and health index that has a higher prevalence in developing countries. Objectives: The present research was conducted to determine the pre-pregnancy risk factors of stillbirth in Iran. Methods: This research is a national population-based case-control study on the risk factors of stillbirth in Iran. A total of 3, 085 women presenting to public healthcare centers of ten provinces/cities of Iran entered the study, including 1, 459 women who their last pregnancy ended in a stillbirth as the case group and 1, 626 women who their last pregnancy ended in a live birth as the control group. Data were collected with a researcher-made questionnaire and were then analyzed by SPSS-19 using the chi-square and the logistic regression tests. P values less than 0. 05 were considered statistically significant. Results: The results revealed a relationship between the last pregnancy ending in a stillbirth and a previous history of stillbirth (OR = 2. 64, CI: 1. 81-3. 85, P = 0. 001), miscarriage (OR = 1. 57, CI: 1. 21-2. 03, P = 0. 001) irregular menstruation (OR = 1. 29, CI: 1. 02-1. 64, P = 0. 029), age over 35 (OR = 1. 58, CI: 1. 17-2. 14, P = 0. 001), low level of education (OR = 3. 50, CI: 2. 30-5. 33, P = 0. 001), and the use of oral contraceptives. Conclusions: There are several risk factors for stillbirth, including a previous history of stillbirth, miscarriage or irregular menstrual cycle, most of which can be controlled through pre-pregnancy training. Educational interventions are, therefore, required to improve the knowledge of womenat childbearing age and preventive measures should be taken to reduce thenumber of stillbirths in pregnant women.