The objective of the research was to validate the AOT for estimation of VO2max in healthy pupils. Subjects included 80 boys and 102 girls selected by purposeful stratified sampling based on age criteria. Subjects participated in both AOT and a criterion test during a one week period. Age sub-groups were 10, 11, 12, 13, 14, 15, 16, and 17 years of age, each consisting of at least 10 volunteers. The criterion measures were directly obtained from gas analyzer outputs during a treadmill workout. Statistical analysis was based on multiple regression analysis. For boys, mean (±standard deviation) of weight, height, maximal heart rate, BMI, and relative VO2max were 55 (±17kg), 163 (±15cm), 201 (±8beat), 20.19 (±3.84kg/m2), 38 (±6ml/kg/min), respectively. The same measures for girls were 46 (±100kg), 156 (±9cm), 199 (±9beat), 18.60 (±2.95kg/m2), 34 (±4ml/kg/min), respectively. The study (P<0.01) the relationship between organizational culture with knowledge management indicates that knowledge management when will be successful performed in organization if appropriate cultural context is provided to this system. Because the guidance of the current wisdom of the organization is popular issue, and depends to staff organizational culture. Therefore organization should provide the appropriate culture for successful performance of knowledge management.showed that there was no significant relationship between age and relative VO2max for girls, whilst it was significant for boys (P £ 0.01). For both boys and girls, increase in BMI was accompanied by decrease in maximal heart rate and VO2max. Calculating formulas were produced in order to estimate VO2max from measures obtained of field AOT or Lab treadmill protocol. Compared to similar studies, produced formulas were scientifically acceptable, and the estimations were sufficiently accurate. High determination coefficients (Min = %45 and Max =%65) and low standard errors of estimate (Min=2.97 and Max=3.78 ml/kg/min) indicated that VO2max estimations by AOT protocols were considerably trustworthy. It is noteworthy that in comparison to adults, estimating formulas of V02max in adolescents generally have less determination coefficients.