Introduction: Toxoplasmosis is Zoonoses among humans and animals with cosmopolitan distribution. Acquired form of the disease often has no symptoms or discomfort of swollen lymph nodes and associated Chorioretinitis. The congenital form of the disease is delivered via the placenta from mother to fetus. Congenital infection may cause abortion or damage to the central nervous system and eye disorders. The aim of this study was to determine toxoplasmosis Seroepidemiologyin serum of suspected patients referring to medical lab, in 2013. Materials & Methods: This study was cross- sectional. After physical examination, of the total cases 712 were diagnosed with suspected toxoplasmosis infection, and were referred for evaluation of serological diagnostic laboratories. Serum samples were collected from patients' in the laboratory using kits of anti-Toxoplasma gondii and Chorus Toxoplasma IgG, IgM antibodies were tested by ELISA method. Data on age, sex and time of the visit and laboratory test results were recorded in the Czech list, and then were analyzed using SPSS software.Results: From 712 sera tested, 649 (91.2%) were female while 63 ((8.8% were male. 171 (24.3%) of the antibody IgG and 25 ((3.5% in terms of IgM in serum were positive. In sex -wise distributed groups 159 female (93%)) and 12 male (7%) tested positive for IgG. and, 24 female (96%)) and 1 male (4%) were IgM positive. Most positive tests (9.5%) were observed in the group aged over 50 years. whereas The lowest percentage of positive tests were in the group with age less than 20 years (20%) and the highest was observed in patients above 60 years (8.47 percent).Conclusion: As a general conclusion, it can be stated that the frequency of specific IgM and IgG antibodies in toxoplasmosis, in the suspected-to-have toxoplasmosis and control groups were not statistically significant. Also, we can conclude that abortion is involved in the development of chronic toxoplasmosis.