In recent years, global climate change and water resources drought have been the most important environmental issues in aquatic life in order to tolerate changes in temperature and humidity. Diapause and anhydrobiosis are the adaptation mechanisms of organisms to survive these changes. The accumulation of sugars and polyol are the common physiological mechanisms among anhydrobiotic organisms. In this study, the change of glycerol contents was investigated. The samples were collected seasonally from two stations and for one year from autumn 2015 to summer 2016. The amount of glycerol was 18. 31, 7. 65, 17. 63 and 23. 21 μ g/mg wet weight for Simuliidae and 8. 19, 7. 36, 30. 35 and 8. 97 μ g/mg wet weight for Tipulidae; and 44. 43, 17. 85, 38. 07 and 11. 13 μ g/mg wet weight for Baetidae during spring, summer, autumn and winter, respectively. In Simuliidae the glycerol contents did not show any significant differences between summer and autumn (P > 0. 05) while it showed significant differences (P < 0. 05) in other seasons. In Tipulidae the glycerol had not significant differences in spring and summer (P > 0. 05) and also showed no significant differences in spring and winter (P > 0. 05) while there were significant differences (P < 0. 05) between autumn and other seasons. In the Baetidae the glycerol contents showed significant differences among different seasons (P < 0. 05). The increasing polyols with low molecular weight associated with diapause and anhydrobiosis in early autumn, when the insect larvae exposed to cold shock and increase the cryoprotectant components and consume them as antifreezing agent in winter. In addition, the insect larvae increase these compounds during spring, which prepare them to survive in high temperature and desiccation during summer. This study illustrated that the insect larvae responses to environment ecological conditions by glycerol content, especially during freezing and desiccation.