مجله دانشگاه علوم پزشکی اراک
سال:1392 | دوره:16 | شماره:6 (پیاپی 75) |تعداد مقالات:14

Background: Saffron (Crocus Sativus) petals consist of, glycosides, flavonoids, and anthocyanins. Due to the increasing use of medicinal plants in treatment of diseases and identifying their side effects on various organs, this study is to evaluate the effects of Crocus Sativus petals on biochemical blood parameters in male rats.Materials and Methods: In this experimental study, 30 male Wistar rats with the mean body weight of 215±15 g were randomly assigned into five groups of 6 animals each. Control group received normal saline (10 ml/kg) and treatment groups received 75, 150, 220 and 450 mg/kg body weight of saffron petals extract, for 14 days. Body weight measured in days 0, 7, and 14. At the end of experiment, serum levels of liver function marker enzymes such as AST and ALT, total proteins, albumin, creatinine, cholesterol and triglyceride were assessed.Results: Compared to control group, ethanol extract of saffron petals significantly increased serum albumin and also significantly decreased levels of serum cholesterol and AST (p<0.05). No significant changes were observed on serum total proteins, creatinine, ALT, triglyceride levels and body weight in control and saffron groups (p>0.05).Conclusion: Increase in levels of albumin and decrease in liver enzymes may indicate the lack of liver damage. Reduction in cholesterol which is probably due to flavonoid extracts of saffron and no effect on body weight can be beneficial in the use of saffron petals.

Background: Exercise is a stimulus for the emergence of HSP and liver enzymes in the blood The purpose of the present study was to investigate the probable changes of HSP70, liver enzymes & Cpk in professional athletes after a training season and participating in skating open world championship.Materials and Methods: 10 elite female skaters were chosen. Eccentric exercise test were performed in three sections (24 hours before the beginning of the training, after six months of training (before participating in Skating World Championship) and 24 hours after the end of world championship). The training program consisted of 26 weeks of training, performed 5 times a week encompassing specialized skate trainings. Blood samples were taken before and after each eccentric exercise test.Results: there was no significant change, in HSP70 concentration in response to eccentric exercise test, in pre exercise period (p=0.8). But, it illustrated a significant increase in after eccentric exercise test, in post exercise period (p=0.03). Moreover, in measuring down, it showed a significant increased in the amount of liver enzymes and Cpk after eccentric exercise test in second and third step compared to first step (p<0.05).Conclusion: the results of the study illustrated that improving an athlete's physical fitness level during training season and matches leads to a significant increase in the protective level of the body (via the production of HSP70), depending also on the ability of the body of the individual in producing that protein. It can also be stated probably individual body fitness level, is an important factor in determining ALT, AST, ALP levels after performing eccentric exercise.

Background: There are evidences indicating that inflammatory mechanisms within the central nervous system contribute to cognitive impairment via cytokine-mediated interactions between neurons and glial cells. BAT1, a member of the DEAD-box family of RNA helicases, appears to regulate the production of inflammatory cytokines associated with Alzheimer’s disease (AD) pathology. In the current study BAT1-22 promoter polymorphism was analyzed in AD and control subjects.Materials and Methods: In this case-control study, genomic DNA from peripheral blood samples of 153 Alzheimer’s patients and 153 healthy controls was extracted using salting-out method. DNA analysis was performed by PCR-RFLP method and p<0.05 was considered statistically significant.Results: After genotyping and statistical analysis the results failed to show any association between BAT1-22 promoter polymorphism and sporadic Alzheimer’s disease.Conclusion: BAT1-22 is not associated with Alzheimer’s disease in Iranian population and so has no effect on predisposition to sporadic Alzheimer’s disease.

Background: Traditionally levels of cholesterol and triglyceride are used to identify individuals at risk of coronary artery disease (CAD). The aim of this study is examining the association of Apo A1 and ApoB with severity of coronary artery disease and whether these parameters are better predictor of CAD.Material and method: This is a cross- sectional study. All patients that referred to ARAK amirallmomenin hospital and enrolled for coronary angiography was entered to study. Before angiography and after gathering informed consent, levels of apo A1 apoB, cholesterol, TG, LDL, VLDL, HDL and FBS were measured. The results of coronary angiography were reviewed by two experienced cardiologist separately. Severity of coronary artery disease involvement was determined by Gensini score (GS), the data were analyzed with statistical methods by SPSS software.Result: There is a statistically significant correlation Between apoB and GS (r=0.127, p=0.047). Logistic regression model showed that among predictors for CAD entered model eg gender, age, cholesterol, TG, HDL, LDL, VLDL, apo A1, ApoB and ApoB/Apo- A1 ratio, only ApoB and gender were proper predictors of coronary artery Disease (CAD) (p=0.002, p=0.001). In comparison with angiography for diagnosis of CAD, ROC analysis represent that using ApoB can be useful test (p=0.047).Conclusion: According to result of this study, using ApoB in addition to conventional parameters for assessing the patient at risk to having CAD would be reasonable and could be an independent risk factor for CAD.