In order to investigate the effect of zinc nano oxide on yield and some physiological characteristics of wheat under drought stress conditions, a factorial experiment was a three-factor factor conducted in a completely randomized design with three replications in University of Mohaghegh Ardebili in 2018. Factors studied i include the first factor of drought stress at three levels (35%, 60% and field capacity and control), the second factor of wheat cultivars (Mihan, Heidari, Sysons and Gasgogen) and the third factor includes ZnO spraying in three levels (no application of ZnO, 0. 5 and 1 g/l ). Foliar application of ZnO was performed in three-leaf stage and three day later drought stress for one week. ZnO foliar application increased the amount of photosynthetic pigments and leaf chlorophyll index, FV / FM, plant length, spike number and grain weight. Drought stress decreased chlorophyll index (3. 73%), F0 (60. 79%), Fv (11. 18%), Fv / Fm (2. 68%), mean plant height (15. 93%), Total dry weight (16. 27%), dry weight (46. 18%) and root fresh (8. 32%) as well as grain yield (15. 95% of grain number and 11. 93% of grain weight). Also, ZnO foliar application and irrigation under control conditions improved chlorophyll index (151. 01%) and photosystem II fluorescence during growing season (78. 62%) and increased wheat grain yield under (338. 38% of grain number and 361. 62% of grain weight) dehydrated conditions. Among the studied cultivars, the highest amount of F0 (235. 5) and Fm (756/44) belonged to Meihan cultivar and the least amount of (204/944), F0 (34. 0), Fm (362. 28) and FV / FM (0. 4585) belonged to Heidari cultivar. Under drought stress, the weight of one thousand grains in Heydari cultivars (155. 35%) and Gasgogen (32. 60%) decreased compared to control conditions. Among cultivars, Mihan was more tolerant to drought stress. In terms of grain yield under normal conditions, Heydari genotype was recognized as the best genotype.