BACKGROUND: Buffalo is one of the most important animals for the source of milk and meat, and it is also used for draught purposes in Bangladesh. Gastrointestinal parasites in the buffalo calves lead to reduced growth and hinder the development of buffalo industry in Bangladesh. OBJECTIVES: The study was conducted to identify the gastrointestinal parasitic load in the buffalo calves at Sylhet district, Bangladesh. METHODS: Fecal samples were collected from one hundred seventy (170) buffalo calves from different areas of Sylhet district and samples were examined using sedimentation, and floatation techniques. A morphological examination of parasitic eggs was performed to identify the parasitic species. RESULTS: Among the 170 calves examined in the present study, 62 (36. 47%) were found positive with different parasites. Two species of gastro-intestinal parasites were identified, among them 26. 47% positive with Neoascarisvitulorum, 5. 88% with Strongyloides sp. and 4. 12% with Strongyle type. The prevalence of gastro-intestinal (GI) parasites in males (44. 12%) was lower than in female (55. 88%) calves. A relatively higher worm load was observed in summer (58. 82%) than rainy (41. 18%) season. The prevalence of Gastrointestinal parasitic infections was significantly higher (P≤ 0. 05) in calves aged 6 months of age (44. 12%) than calves of 6-12 months of age (32. 35%) followed by 13-18 months of age (23. 53%). EPG (egg per gr of feces) ranged from 50-1950. CONCLUSIONS: The present study showed that the prevalence of GI parasites in buffalo calves in Sylhet is relatively higher regardless of age, sex, and seasons. Data generated through this study will help to adopt necessary measures to control the GI parasitic infections in buffalo calves in developing countries like Bangladesh.