Introduction: Exclusive breastfeeding protects infants from diseases in the first 6-months of life. Awareness and attitude affect breastfeeding performance. This study assessed maternal awareness and attitude toward breastfeeding in Alzahra Hospital, a baby friendly, in Rasht, Iran. Objectives: To determine knowledge and attitudes of mothers on various aspects of breastfeeding and factors related to them among mothers under postpartum, Alzahra educational, medical and research center. Materials and Methods: This descriptive cross-sectional study was conducted on 384 post-natal mothers. It was done by sampling and simple random in post-partom unit of Alzahra Hospital, Rasht. A questionnaire consisting of 3 main parts; demographic information, knowledge and attitude, was used in the study. Attitude and knowledge scores were also calculated. Data were analyzed by Man-Whitney, Kurskal-Wallis and Spearman Correlation Coefficient tests, using SPSS-software in version21. Results: In this study, 32. 6% had good levels of knowledge about breastfeeding and majority of them (39. 1%) had an average knowledge. All of mothers had positive attitude, but 20. 3% of mothers had more positive attitude and most of individuals (67. 2%) were in the intermediate state and the rest had a more negative attitude. In the multivariate analysis in the regression logestic model attitude status (P<0. 0001), experience of breastfeeding(P=0. 006), breastfeeding training (P=0. 004), income (P=0. 03) and mother’ s job (P=0. 001) were anticipating factors of high knowledge, and the status of knowledge(P=0. 043), mothers’ education(P=0. 004), natural delivery compared to cesarean section (P=0. 008), type of infant’ s feeding (P=0. 0001) were anticipating factors related to positive attitude status. Also, a significant poor positive relationship was found between age and the score of knowledge (r=0. 158, P=0. 002) and attitude (r=0. 128, P=0. 062). Conclusion: A significant number of mothers had moderate knowledge about breastfeeding, which necessitates intervention programming, especially for mothers with low levels of education.