Journal of Isfahan Medical School
Year:2014 | Volume:32 | Issue:292|Papers Count:7

Background: In this paper, we investigated the performance of some different classifiers for prediction metastasis in breast cancer.Methods: For this purpose we used the DNA microarrays of primary breast tumors of 78 young patients. Among these patients, 34 patients had developed distant metastases within 5 years (poor prognosis group) and 44 patients formed good prognosis group. For analysis, we applied three different classifiers include Support Vector Machine (SVM), StepWise Linear Discriminant Analysis (SWLDA) and K-Nearest Neighbors (KNN) classifier. Each of these classifiers used 231 selected genes as an input feature vector and their performance was estimated by using leave one out (LOO) method to classify patients into two groups namely, good and poor prognosis.Findings: The best results were obtained by SVM with linear kernel. This classifier achieved a sensitivity and specificity of 84% and 82% respectively for metastasis prediction.Conclusion: Our findings provide a strategy to specify patients who would benefit from adjuvant therapy.

Background: Testicular adrenal rest tumors (TART) is there unspecified adrenocortical cells in the testis of patients with Congenital Adrenal Hyperplasia (CAH) and is similar to the hyperplastic adrenal glands and make testicular tumors that are known as Testicular adrenal rest tumor. These tumors are found frequently in adult man patients with CAH and are usually benign But due to tumor site in testicles, can lead to seminiferous tubule obstruction and gonadal dysfunction and infertility, therefore, early diagnosis and treatment of tumors is important. Due to the absence of exact statistics and studies to determine relative frequency Testicular adrenal rest tumor in patients with CAH and importance of early diagnosis of tumors in pediatric patients with CAH, This study was performed to determine relative frequency of testicular adrenal rest tumor in children with CAH.Methods: This study was a descriptive study that was performed in Al-azahra hospital in 1390. This study was performed on 44 patients with CAH under 20 years old. Height and weight and blood pressure was measured for each patient, also exact examination of testis and scrotum was done to diagnose of palpable mass. For all patients, ultrasound of testis was performed by an experienced radiologist. Assessment of patients was based on patient height and weight and serum level of 17 – hydroxyl progesterone. Finally, relative frequency of testicular adrenal rest tumor was evaluated in patient and its corelation with serum 17–hydroxy progesterone and dosage of medication and disease control rate and duration of disease was evaluated. The obtained data were analyzed by SPSS-18 software.Findings: The ultrasound exam results showed, TART was reported in 2 patients (4.5%) that in one of them, bilateral tumors was reported in the upper pole of both testis. and another tumor was visible in the Mediastinum of left testis. Both patients had type of salt loss. The average tumor size was 14 mm. None of the patients found palpable mass in the examination. Both patients with TART were in the age group 20-15 years old and patients with TART were under hormonal control and appropriate treatment. Serum levels of 17-hydroxy progesterone was less in patients with (TART) than patients without (TART). Any of 2 cases had impaired gonadal function.Conclusion: Testicular adrenal rest tumor (TART) occurs usually in severe forms of the disease (type of salt loss). Two cases with tumors (TART) in our study, were in the age group above 15 years old. It can be shown the correlation between duration of disease and prevalence of tumor. None of two cases found palpable mass in the examination. This can indicate that examination has not enough accuracy to detect tumor and is recommended for all patients, ultrasound be performed periodically.

Background: This study has been designed to predict whole-body fat mass by various anthropometric indices (waist circumference (WC), waist-to-height ratio (WHtR), hip circumference (HC), waist-tohip ratio (WHR) and body mass index (BMI)). Cost and radiation dose reduction are the advantages of this prediction compared to whole body DXA scan.Methods: For this study, whole-body composition was measured with dual-energy X-ray Absorptiometry (DXA) for 143 adult patients who referred to Isfahan Osteoporosis Diagnosis Center. Values of weight, height, waist and hip circumferences were measured and BMI, waist-hip ratio and waist-to-height ratio was calculated. Datasets were split randomly into two parts, the derivation set with 100 subjects and validation set with 43 subjects. Multiple regression analysis with back ward stepwise elimination procedure was used for derivation set and then the estimates were compared with the actual measurements.Findings: Using multiple linear regression analyses, the best equation for predicting whole-body fat mass (R2= 0.808) included BMI and gender.Conclusion: The present study showed that BMI is the best anthropometric predictor of whole-body fat mass (adjusted R2 = 0.680 and SSE = 999.42).

Background: Brucella melitensis infection is still a major health problem for human and cattle in developing countries and the Middle East. Cell-mediated immunity is the dominant immune response required for protection against brucellosis. So the identification of new candidates which can induce cell-mediated immunity are demanded. Proliferation assay is one of methods is used to evaluate the potential of an antigen to generate memory Tcell. In the present study, the proliferation of speloncyets obtained from immunized mice was evaluated when they were challenged by recombinant Omp 31 (rOmp 31) in vitro.Methods: Mice were immunized by PBS, rOmp 31 and formalin-killed B. melitensis Rev.1 with Freund’s adjuvant. At day 30 after the last immunization, spleens were removed and homogenized. Purified rOmp31 (2.5 and 5mg/mL) was added to 2´105 spleen cell and then the speloncytes were incubated at 37 ◦C in 5% CO2 atmosphere. After 48h, XTT and MTT assays were used to show the splenocyte proliferation. Findings: The data showed that splenocytes of mice immunized with rOmp 31 or Rev.1 started to proliferate after stimulation by rOmp 31. There was a significant difference between SIs obtained from rOmp 31 and Rev groups compared to PBS groups (P<0.01) and (P<0.05), respectively.Conclusion: Based on our finding, rOmp 31 could generate a good memory cellular immune response. In addition, the method described here to study the speloncyte proliferation using XTT is a simple and efficient method.

Background: Ectopic pregnancy is a common serious problem with high morbidity percentage and possibility of maternal mortality. We can diagnose ectopic pregnancy earlier than the clinic will be present and this will give us the possibility to apply the best treatment with fewer complications. Quantity b-hCG measure, ultrasound examination inform us for the diagnose of ectopic pregnancy and surgical treatment can be avoided. The treatment will be determined by combination of clinical symptoms, ultrasound examination and b-hCG levels.Methods: we were treated (Semi-empirical) with systemic methotrexate therapy at the Department of Obstetrics and Gynecology, EMAM REZH Hospital in kermanshah. The patients were treated with either of the two regimens: a) Single dose regimen: 50 mg/m2 of intramuscular MTX (60 cases) b) Multiple dose regimen: 4 doses of 1 mg/kg of IM MTX (60 cases). Serum beta-hCG value at initiation of treatment.Findings: The overall success rate of MTX management for an ectopic pregnancy was76% for single and multiple dose regimen was 96.7%. THE rupture of EP mass was 3.3% in multiple dose and 10% in single dose during of treatment.Conclusion: multiple dose of methotrexate therapy is an effective than single dose safe treatment.