JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)
Year:2014 | Volume:32 | Issue:294|Papers Count:5

Background: Wash the burn wound is one of the key factors that to achieve this goal should be appropriet and effective dilution of disinfectant used. We have used various dressings for burn wound healing. Therefore, the purpose of this study was to determine the effects of using hypochloride and betadine dressings on burn wound healing done.Methods: Patients aged 18-45 years 20-45% who are hospitalized whit burns were treatmented hypochlorite and povidone were placed in two groups. Both groups had a bandaged face, and wound dressing daily until complete healing was achieved. When the wound is pink and translucent appearance, no spatter and has been lining the wound healing was considered. Time require healing of the graft and the graft area were declared by the surgeon and recorded.Findings: In this study 50 patients had burns of criteria. Fisher’s exact test showed that the nead to graft in hypochlorite was significantly lower than Iodine group (P=0.04).Independent t-test showed that the average extent of graft in hypochloride group was significantly lower than Iodine group.Conclusion: Results showed that the use of hypochlorite reduces the duration of hospitalization and treatment costs and reduces healing time and the need for graft.

Background: Insulin resistance is a condition in the form of the lack of sensitivity of the tissues for removing glucose. Colorectal polyps with the histology basis has the risk in transforming to the malignant neoplastic form. There are some uncontrollable risk-factors that influence the immergence colorectal neoplasms, though some of them are relatively controllable, among which the insulin resistance could be named. The aim of this investigation is analyzing the relation between insulin resistance and affecting to colorectal neoplasms in the people with 50 years of age or more.Methods: This study was done in descriptive-analytic cross-sectional study method on 200 patients with 50 years of age and older, with indications of colonoscopy and without the risk factors such as family history of colon, affecting to cancer, intestinal inflammation, anemia, rectal bleeding and so on. BP, waist circumference, height and weight of patients were measured. Their HDL, FBS, TG and plasma insulin were controlled and then, they underwent colonoscopy. In case of observing any abnormal disorders, the samples were sent for pathological controls. Finally, the recorded data was analyzed by SPSS21 software.Findings: The results of the research show that the frequency of colorectal polyps are more in 50 year old people affected by insulin resistance syndrome (P value=0.007). There are significant relations between the affected people to insulin resistance syndrome, the people with raised fasting blood suger, the ladies with low HDL and metabolic syndrome with adenoma with advanced pathology (P<0.05), but no significant relation exists in cases with hypertension, age, sex, obesity and hypertriglyceridemia (P>0.05).Conclusion: This study shows that the frequency of colorectal polyps, especially the advanced pathology is observed more in the people affected to insulin resistance, diabetic people or the people who are susceptible to diabetes. It is recommended for the mentioned patients to have investigations of colonoscopy in younger ages to prevent deaths due to colorectal cancer. It is also necessary to have more investigations in this regard.

Background: Surgery, radiation therapy, drug therapy or a combination of these methods can be used for the treatment of malignancies. Drug therapy in cancer patients includes of chemotherapy, Biotherapy and use of monoclonal antibodies against antigens of malignant cells. One of the compounds that was used as drugs in chemotherapy against malignant cells, are heavy metal complexes. The present study aimed to assess the effects new anticancer palladium complexes have been performed on esophageal cancer cells.Methods: New palladium complexes, namely [(phen) Pd (m-al-bis-dtc) Pd (phen)] (NO3)2 (where alkylenebisdithiocarbamate, al-bis-dtc= propylenebisdithiocarbamate (pn-bis-dtc, 1); butylenebisdithiocarbamate (bu-bis-dtc, 2); octylenebis- dithiocarbamate (oc-bis-dtc, 3) and phen=1,10-phenanthroline) was synthesized in the laboratory of Chemistry, University of Sistan and Baluchestan. In order to investigate the anticancer effects of three new complexes compared to cisplatin on esophageal cancer cell line (KYSE-30), cytotoxicity was examined through MTT and clonogenic assays. Ethidium bromide/acridine orange (EB/AO) staining was used for apoptotic and necrotic cells detection.Findings: The results of MTT and clonogenic assays showed that the IC50 value obtained from cells treated with complexes 1, 2 and 3 were much lower than cisplatin and also Ethidium bromide/acridine orange staining demonstrated that three new Complexes apply its cytotoxic effect via apoptotic pathway. Comparing the number of colonies formed after treatment with various concentrations of palladium complexes, showed significant differences compared with control. (P<0.001).Conclusion: Findings of this study showed that anti-tumor effect of three new Complexes on KYSE- 30 cell line were higher than that of cisplatin. And even the use of low concentrations of palladium complexes can reduce the number of colonies and induces cell death by apoptosis and if it can do further researches later be used as an alternative drug in the treatment of esophageal cancer.

Background: Obesity and sedentary lifestyle are known as important risk factors of coronary artery disease. The prevalence of obesity has increased among both men and women in the world. Therefore, the present study tried to evaluate the effectiveness of a cardiac rehabilitation program on functional capacity and body mass index (BMI) in obese and nonobese women with coronary artery disease.Methods: In an observational study during 2007-13, we evaluated a total of 205 women with coronary artery disease who referred to the cardiac rehabilitation unit of Isfahan Cardiovascular Research Institute, Isfahan, Iran. BMI and functional capacity of each patient were assessed before and after the program. The patients were categorized as obese or non-obese based on their BMI. All participants completed the full course of the program. Data was analyzed by independent t-test and paired t-test in SPSS 15.Findings: Our finding showed that an 8-week cardiac rehabilitation program had significant effects on functional capacity in obese and non-obese female patients (P<0.01 for both). The program also resulted in BMI improvements in both groups (P<0.01 for both). Comparing the changes in the two groups did not reveal any significant differences in functional capacity. However, the two groups were significantly different in terms of BMI changes.Conclusion: Cardiac rehabilitation programs are a major step in restoration of functional capacity and improvement of BMI in obese and non-obese women with coronary artery disease.

Background: Nowadays, in many studies related to the treatment of various cancers, toxic compounds were targeted against cancer cells. One of the most effective compounds is Pseudomonas exotoxin A. The purpose of this study is to investigate the expression, purification, and in vitro evaluation of a short form of the toxin in a prokaryotic expression system.Methods: The short form of the toxin (PE38) was amplified by PCR using primers containing HindIII and NdeI restriction enzyme sites from pUC57-PE38. The PCR product was digested and subcloned in the pET-26b expression vector. The expression vector was separately transformed into the BL21 (DE3), BL21 (DE3) plys S and Rosetta E.coli strains. Recombinant bacteria were cultured and induced and the resulted PE38 protein purified using metal affinity column chromatography. The toxicity effect of PE38 protein was assessed on HUVEC and 293KDR eukaryotic cells.Findings: The gene was successfully cloned into the expression vector and the accuracy of construct was confirmed by restriction map analysis and sequencing. The expression was found more in BL21(DE3) than the other strains. The toxicity effect was observed at the same level for both HUVEC and 293KDR cells.Conclusion: The lethal dose of truncated toxin is more than the previous study (1000-fold), where the targeted vascular endothelial growth factor (VEGF121) was fused to the truncated toxin.