Armaghane Danesh
Year:2014 | Volume:18 | Issue:11 (83)|Papers Count:11

Background & aim: Royal jelly, secreted by worker bees, has different biological activities on cells and tissues. The aim of this study was to evaluate the effects of royal jelly on peripheral blood mononuclear cells and on the tumor category of K562 cell line.Methods: In the present experimental study, three subjects were selected separately with three repetitions. K562 (104 cells) and PBMC (105 cells) with different concentrations of royal jelly (5, 10, 25, 50 and 100 mg/ml) were cultured under standard conditions for 48 and 72 h separately. The fatality rate on PBMC cells and K562 cancer cells was evaluated by using MTT (Tetrazolium Dye-Reduction Assay). The number of viable cells in PBMC that were exposed for 48 hours with Royal Jelly was evaluated by trypan blue staining. Data were analyzed by ANOVA.Results: The royal jelly had no cytotoxicity effect on PBMC cells but at concentration of 50 and 100 mg/mL the cytotoxicity effect were observed on k562 cells whereas, at 10 and 25 mg/ml the number of PBMC viable cells increased.Conclusion: Due to the lack of lethality of royal jelly on PBMC cells and PBMC cell viability and an increase in the fatality rate of cancer cells in the future, royal jelly can be used as a potential candidate for treatment of leukemia.

Background & aim: Soy contains the largest concentration of isoflavones, they are structurally similar to estradiol and mimic its effects. The purpose of this study was to evaluate the effect of soy milk on thickness of cerebral cortex and hippocampus in newborn ovariectomized rats.Methods: The present experimental study was conducted on 30 newborn female rats. In day 7, 20 rats were randomly chosen and ovariectomy surgery was performed on them. Then the rats were divided to six groups of five rats each: control group, ovariectomized rats (no treatment), sham group and 3 groups of ovareictomaized rats treated by soy milk in doses 0.75, 1.5 and 3 ml/kg/day.In day 60, the brains of all rats were dissected and histological studies for determination of thickness of cerebral cortex and hippocampus were done. The collected data were analyzed with one-way ANOVA and Tukey tests.Results: Our results indicated that low estradiol following ovariectomy decreased thickness of cerebral cortex and hippocampal regions and soy milk in high dose increase the levels of estradiol, thickness of cerebral cortex and thickness of hippocampal regions.Conclusion: soy milk can compensate the effect of ovariectomy on thickness of cerebral cortex and hippocampal regions

Background & aim: Tichomoniasis is a common sexually transmitted disease (STD) caused by a protozoan parasite called Trichomonas vaginalis. Metronidazole, with vast side effects, is the main treatment for this infection. The aim of this study was to investigate the effect of the extract fractions of Taxusbaccata, on Trichomonas vaginalis in cultured medium.Methods: In the present study, asetonia dichloromethane extract of Taxusbaccata and hydroalcoholic fractions of 60% and 90% were collected and then dried. Their anti-trichomonas effect at doses of 200, 300, 400 and 500 mg/ml at 24, 48 and 72 hours on the TYIS33 culture media were studied. Data were analyzed using ANOVA test..Results: The crude extract and fractions of 60% at different times at concentrations of 200, 300, 400 and 500 mg/ml showed higher anti-parasitic effects than 90% (p<0.05). At concentration of 200 μg/ml of extract of 60% which causing 100% inhibition growth but the fraction of 90% showed 60% of inhibition growth.Conclusion: The effect of Taxusbaccata on Trichomonas may be a good alternative candidate for metronidazole.

Background & aim: Changes of alkaline phosphatase activity is one of the symptoms of many diseases. The aim of this study was to evaluate the effect of two types of Daphne, Betulin and Betulinic acid, on alkaline phosphatase activity in K562 and MCF-7 cell lines, respectively.Methods: In this study, 106 cancer cell lines of K562 and MCF-7 were cultured in presence of 5% carbon dioxide at 37 oC. at doses near the IC50. The viability of cells, inside and outside alkaline phosphatase activity and the amount of total protein in each treatment were studied. The collected data was analyzed with a multivariate analysis of variance (Nested Design) and Dunnett test.Results: The intracellular alkaline phosphatase activity of the cells showed different behavior compared to the extracellular alkaline phosphatase activity (p<0.01). The highest increase of alkaline phosphatase activity in two cell lines (K562 and MCF-7) were 339% and 236% which was related to the treatment by macronata daphne.Conclusion: Unexpected increase in intracellular alkaline phosphatase activity in D. mucronata, D. oleides, Betulin, and Betulinic acid treatment may be due to changes in the composition of plasma membrane component and an increase the non-connected membrane of the protein which is due to the creation of more active proteins.

Background & aim: Myocardial infarction is one of the most common cardiovascular diseases. Streptokinase is used in patients with myocardial infarction with ST-segment elevation. The aim of this study was to compare the side effects of intravenous streptokinase in acute myocardial infarction patients in different age groups.Methods: In the present analytic descriptive study, the samples included 100 eligible patients which received intravenous streptokinase. The research instrument was a questionnaire consisting of two parts: the first part was used to measure the underlying data and the second part to evaluate the side effects of intravenous streptokinase. Data were analyzed by chi-square statistical tests.Results: There were no significant adverse events between intravenous streptokinase and different age groups (P>0.05). The most common side effects of this drug were cardiovascular complications in different age groups.Conclusion: the use of streptokinase intravenous drug in different age groups is associated with risk of cardiovascular complications.

Background & aim: Green pepper and parsley have high antioxidant properties due to flavonoids and polyphenolic compounds. The aim of this study was to investigate the hepatoprotective effects of hydroalcoholic extract of green pepper and parsley on the toxicity induced by carbon tetrachloride in rats.Methods: In the present experimental study, 48 male Wistar rats were divided into six groups of eight.The first group (negative control) received olive oil, the second group receiving tetrachloride carbon and olive oil in equal proportions. The third and fourth groups (treatment groups) were treated with the tetrachloride carbon and olive oil equally and then exposed to extract of parsley and green pepper to the amount of 200 mg/kg separately. The fifth and six groups were treated with tetrachloride carbon and olive oil equally, and then separately exposed to 400 mg/kg of parsley and green pepper extract. The amount of total phenol and flavonoid samples and antioxidant activities were assessed by different methods. The collected data were analyzed by One-way ANOVA tests followed by Tukey test.Results: Intraperitoneal injection of 202, 191 and 183 In. U/L of carbon tetrachloride to positive control increased the activity of alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase, respectively. The injection of carbon tetrachloride increased total bilirubin at the rate of4.8 mg/dl and also decreased protein and albumin at rate of 5.1 and 2.6 g/dl. Mqdartvtal respectively 1/5 and 6/2 g, was dL (p<0.001). In the groups treated with the extract the activity of alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase in the third to sixth groups were 121, 115 and 109, 130, 121 and 121, 112 109 and 98, 118, 114, and 119 respectively. The values of bilirubin, total protein and albumin, in the III group were: 3.8, 5.5 and 2.8, Group IV: 3.9, 5.3 and 2.7, the V: 3.6, 5.8 and 3.1, the VI: 3.8, 5.6 and 3, respectively. Finaly, the amount of total bilirubin, total protein and albumin of groups’ three to sixth were obtained 3.8, 0, and 2.8, 3.9, 0.2 and 2.7, 3.6, 0.8 and 2.6, 3.8, 0.6, and3 respectively.Conclusions: Administration of green pepper and parsley extract at doses of 200 mg and 400 mg/kg could have a protective effect against liver damage induced by carbon tetrachloride.

Background & aim: It has been estimated that about 30-40 percent of all cancers could be prevented by diet and lifestyle. In the present study, associations between food groups and some nutrients were studied in adult glioma.Methods: In the present hospital-based case-control study which took place in Tehran, Iran, from 2010 to 2012, socio-economic information, demographics, lifestyle factors, health and dietary intakes of 128 patients with glioma as cases, and 256 healthy controls, were collected through interviews and questionnaires. Logistic regression was used to estimate odds ratios. SPSS version 19 was used for all statistical analyses.Results: After adjusting for potential confounders, inverse associations between calcium intake and adult glioma and vitamin C were observed. (Highest tertile versus lowest: OR=0.20, 95% CI=0.87-0.46, P for trend=0.001) and vitamin C (OR=0.34, 95% CI=0.15-0.76, P for trend=0.002). In addition, we observed elevated ORs for highest vs. lowest tertile of cholesterol intake (OR=2.78, 95% CI=1.29-5.99, P for trend=0.061). We observed no significant associations with adult glioma for intakes of total fat, carbohydrate, protein, vitamin A, vitamin E and beta carotene.Conclusion: the results showed that intake of calcium and vitamin C may possibly prevent glioma in adults.

Background & aim: The role of family in shaping affective and cognitive characteristics of children, especially girls, who the future health of community depends on their mental health, is evident. The purpose of this study was to determine the predictive power of family cohesion and flexibility on self-esteem and happiness of children in high school girl students in Shiraz.Methods: In the present correlational study design, 303 cases of Shiraz secondary girl students who lived with their parents were chosen through the multistage random cluster sampling method. They responded to questionnaires for consistency of family, positives flexibility, Cooper Smith Self-esteem and Oxford Happiness scale. The data were analyzed by Pearson correlation and multiple regressions. Results Family consistency had significant positive power predictive for criterion variables self-esteem and happiness (p<0.05), whereas the flexibility had no such significance.Conclusion: Quality of operation of parents, communication and interaction in family life is one of the best determinants of behavior, health and well-being of children, including their self-esteem and happiness.