In order to study the effect of different amount of nitrogen and different time of application on yield and nitrate content in potato an experiment was conducted in 2006 in Raziabad farm. Treatments imposed were as follows: Five levels of nitrogen (0, 90, 180, 270 and 360 kg ha-1) as a main factor and four times of application T1: one third at planting, one third at emergence and one third before flowering, T2: one half at emergence and one half before flowering, T3: two third at emergence and one third before flowering, and T4: one third at emergence, one .third twenty five days later and one third before flowering. The experiment was a: complete randomized block with three replications. At harvest plant height, number of tubers per plant, average tuber weight, yield per plant, average tuber dry weight and the amount of nitrate accumulated in the tuber were measured. Results indicated that with increased nitrogen plant height, and the amount of dry weight increased but tuber number decreased both amount and time of application had pronounced effect on yield and average tuber weight. Highest yield obtained with T4 (180 kg N ha-1), however yield decreased when higher amount on nitrogen was used. The amount of nitrate accumulated in the tubers was affected by nitrogen and time of application. Highest amount of nitrate corresponded with 360 kg N ha-1 at T2 and T3.