Introduction: In this study we investigated morphological structure of Cyprinidae fish, kutum (Rutilus frisii kutum Kamenskii, 1901) in Caspian Sea using truss network system.Methods: Samples were collected from two beach seine and gill net fisheries cooperation's located in two main fishing areas of this species in the western (Bandare-Astara) and the eastern (Bandare-Kiyashahr) shore of Guilan province. Truss distance as well as eye diameter, interorbital, total length, fork length width of 23 samples were measured in ichthyological research laboratory. Findings: Our results by using Statistical analysis (One-way ANOVA) for morphological characteristics revealed that 14 morphological traits showed significant differences that showed relatively high phenotypic diversity among samples in the two kutum populations. Morphological characters including: total length, standard length, eye diameter and landmark distances including: dorsal first spine and pectoral fin, snout and pelvic fin, the upper branch of caudal fin and the lower branch of caudal fin, the first upper branch of caudal fin and the last soft branch of pelvic fin, the last soft branch of dorsal fin and the first spin of pelvic fin, the last soft branch of dorsal fin and the upper branch of caudal fin, the first lower branch of caudal fin and the last soft branch of pelvic fin, the posterior part of pelvic fin and the last soft branch of dorsal fin, the first upper branch of caudal fin and the first spin of pelvic fin, snout and basement membrane gill and the first spin of dorsal fin and basement membrane gill. In principle, any measured distances between landmarks may serve as characters for morphometric analyses. We described methodologically a geometric protocol for cyprinid fish species morphological character measurements, the truss network system, which enforces systematic coverage of the form and exhaustively and redundantly archives the landmark configuration.