Study on 6-gingerol in ginger (Zingiber officinale Roscoe. ) complete extract and its molecular effects on HTC-116 colorectal cancer cell line

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SOLEIMANI T.; EBRAHIMI A.; MAHJOUBI F.; SADEGHI M.

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS RESEARCH Year:2022 | Volume:38 | Issue:3 (113) Page(s): 438-449

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Title

Study on 6-gingerol in ginger (Zingiber officinale Roscoe. ) complete extract and its molecular effects on HTC-116 colorectal cancer cell line

Author(s)

SOLEIMANI T. | EBRAHIMI A. | MAHJOUBI F. | SADEGHI M. | Issue Writer Certificate

Keywords

Ginger (Zingiber officinale Roscoe.)

6-gingerol

Colorectal cancer

HCT-116 cell line

Abstract

Ginger (Zingiber officinale Roscoe. ) is a spicy medicinal plant with antioxidant, antitumor, and anticancer properties. The present study was conducted to evaluate the effects of ethanol extract of fresh ginger rhizome on inhibiting HCT-116 colon cancer cells and the expression of TGFBR2 and DDC genes as tumor suppressor genes and β,-Actin gene as reference gene. HPLC analysis was used to identify and measure the amount of 6-gingerol in the extract. Toxicity of different concentrations of the complete extract (0, 10, 50, 100, 150, 200, 250, 300, 350, 400, and 500 μ, g. ml-1) on the HTC-116 cell line was investigated using the MTT test, 16 and 24 hours after the start of the test (at 16 and 24). The expression of TGFBT2, DCC, and β,-Actin genes was assessed by RT-PCR after treatment with concentrations of 150 and 300 μ, g. ml-1 of the complete extract at 16 and 24 hours. The amount of 6-gingerol was obtained 86. 2 ±,2. 03 mg per 100 g dry weight of ginger ethanol extract powder based on the HPLC results. The MTT test results showed that IC50 was 80. 44 at 16 h and 473. 19 at 24 h. Cell mortality was significantly increased at concentrations of 150 and 300 μ, g. ml-1 of the extract. Also, expression of the TGFBT2 and DCC genes increased at 150 μ, g. ml-1 at both 24 and 16 hours significantly (P<0. 01). The present research proved the ginger extract effect on tumor inhibitory genes induction in HCT-116 colon cancer cell line.

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APA: Copy

SOLEIMANI, T., EBRAHIMI, A., MAHJOUBI, F., & SADEGHI, M.. (2022). Study on 6-gingerol in ginger (Zingiber officinale Roscoe. ) complete extract and its molecular effects on HTC-116 colorectal cancer cell line. IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS, 38(3 (113) ), 438-449. SID. https://sid.ir/paper/1033072/en

Vancouver: Copy

SOLEIMANI T., EBRAHIMI A., MAHJOUBI F., SADEGHI M.. Study on 6-gingerol in ginger (Zingiber officinale Roscoe. ) complete extract and its molecular effects on HTC-116 colorectal cancer cell line. IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS[Internet]. 2022;38(3 (113) ):438-449. Available from: https://sid.ir/paper/1033072/en

IEEE: Copy

T. SOLEIMANI, A. EBRAHIMI, F. MAHJOUBI, and M. SADEGHI, “Study on 6-gingerol in ginger (Zingiber officinale Roscoe. ) complete extract and its molecular effects on HTC-116 colorectal cancer cell line,” IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS, vol. 38, no. 3 (113) , pp. 438–449, 2022, [Online]. Available: https://sid.ir/paper/1033072/en

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