ISOLATION OF Δ5-3β-HYDROXYSTEROID DEHYDROGENASE INVOLVED IN THE BIOSYNTHETIC PATHWAY OF CARDENOLIDES AND ITS EXPRESSION LEVEL UNDER THE INFLUENCE OF SALICYLIC ACID AND METHYL JASMONATE ELICITORS IN FOXGLOVE (DIGITALIS NERVOSA L)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MAROUFI A.; SALIMI V.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2016 | Volume:25 | Issue:1 Page(s): 97-110

Download Full-Text

Persian Verion

View:

1,025

Download:

Cites:

Information Journal Paper

Title

ISOLATION OF Δ5-3β-HYDROXYSTEROID DEHYDROGENASE INVOLVED IN THE BIOSYNTHETIC PATHWAY OF CARDENOLIDES AND ITS EXPRESSION LEVEL UNDER THE INFLUENCE OF SALICYLIC ACID AND METHYL JASMONATE ELICITORS IN FOXGLOVE (DIGITALIS NERVOSA L)

Author(s)

MAROUFI A. | SALIMI V. | Issue Writer Certificate

Keywords

CARDIAC GLYCOSIDESQ1

FOXGLOVEQ1

METHYL JASMONATEQ1

SALICYLIC ACIDQ2

Δ5-3β-HYDROXYSTEROID DEHYDROGENASEQ1

Abstract

Foxglove plants are important sources of medicinal CARDIAC GLYCOSIDES compounds such as digoxin and digitoxin, which are used for treatment of congestive heart failure and cardiac arrhythmia. Digitalis has several valuable species but the only species native to Iran is digitalis nervosa. Considering the importance of study on native plants in order to be used in pharmaceutical industry this research was carried out. One of the key genes in the biosynthesis of digitalis cardenolide in plants is3b-HSD, which encodes the enzyme 3b-HSD with important role in early stage of the cardenolide biosynthesis pathway. Purpose of the present study was to determine nucleotide sequence of 3b-HSD, phylogenetic analyses and its expression under influence of SALICYLIC ACID and METHYL JASMONATE elicitors in different tissues. Results revealed that a sequence encoding the enzyme show very close affinity to Digitalis lanata. In addition expression pattern of 3b-HSD in the transcript level revealed that a significant expression level was detected in leaf tissue, and also under treatment of METHYL JASMONATE and SALICYLIC ACID the expression level raised. Findings of our study could be used to increase cardenolide through metabolic engineering and genetic manipulation.

Cites

No record.

References

No record.

Cite

APA: Copy

MAROUFI, A., & SALIMI, V.. (2016). ISOLATION OF Δ5-3β-HYDROXYSTEROID DEHYDROGENASE INVOLVED IN THE BIOSYNTHETIC PATHWAY OF CARDENOLIDES AND ITS EXPRESSION LEVEL UNDER THE INFLUENCE OF SALICYLIC ACID AND METHYL JASMONATE ELICITORS IN FOXGLOVE (DIGITALIS NERVOSA L). IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 25(1), 97-110. SID. https://sid.ir/paper/104353/en

Vancouver: Copy

MAROUFI A., SALIMI V.. ISOLATION OF Δ5-3β-HYDROXYSTEROID DEHYDROGENASE INVOLVED IN THE BIOSYNTHETIC PATHWAY OF CARDENOLIDES AND ITS EXPRESSION LEVEL UNDER THE INFLUENCE OF SALICYLIC ACID AND METHYL JASMONATE ELICITORS IN FOXGLOVE (DIGITALIS NERVOSA L). IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2016;25(1):97-110. Available from: https://sid.ir/paper/104353/en

IEEE: Copy

A. MAROUFI, and V. SALIMI, “ISOLATION OF Δ5-3β-HYDROXYSTEROID DEHYDROGENASE INVOLVED IN THE BIOSYNTHETIC PATHWAY OF CARDENOLIDES AND ITS EXPRESSION LEVEL UNDER THE INFLUENCE OF SALICYLIC ACID AND METHYL JASMONATE ELICITORS IN FOXGLOVE (DIGITALIS NERVOSA L),” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 25, no. 1, pp. 97–110, 2016, [Online]. Available: https://sid.ir/paper/104353/en

Related Journal Papers