Effects of different growth regulators, gelling agents and carbon sources on in vitro growth of Berberis vulgaris var. asperma

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SAADAT Y.A.; ABBASI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2018 | Volume:26 | Issue:1 (51) Page(s): 121-131

Download Full-Text

Persian Verion

View:

568

Download:

Cites:

Information Journal Paper

Title

Effects of different growth regulators, gelling agents and carbon sources on in vitro growth of Berberis vulgaris var. asperma

Author(s)

SAADAT Y.A. | ABBASI A. | Issue Writer Certificate

Keywords

BAQ2

DKW nutrient mediumQ2

Fars provinceQ2

PhytagelQ2

rootingQ2

sucroseQ2

Abstract

Berberis vulgaris var. asperma, is a medicinal plant of Iran and lack of healthy vigorous seedlings is the main limitation of BArberry cultivation. This research was carried out to develop a suitable technique for mass micropropagation of the species. Effects of different plant growth regulators, different gelling agents and different carbon sources on in vitro growth of BArberry were studied in several experiments. BAsed on the results of the experiments, nodal segments containing 1-2 axillary buds of semi-hardwood shoots were the best explants for establishment of clean in vitro cultures. Surface disinfection of plant materials originated from adult trees was performed with immersion in 2 gl-1 of Benomyl solution for one hour, then 1% solution of commercial bleach for 15 minutes and rinsed three times with sterile distilled water. Explants cultured on media solidified with Phytagel were significantly better than the media with DifcoBActo agar and Agargel for shoot production. Addition of 3 gl-1 activated charcoal resulted in control of nutrient medium and explants browning, improvement of survival and in vitro growth of the explants. DKW medium containing 2 mgl-1 BA, 0. 5 mgl-1 IBA, 2 mgl-1 GA3, 3 gl-1 activated charcoal and solidified with 2. 5 gl-1 Phytagel was optimum for in vitro growth of BArberry and recommended for shoot multiplication of the species. rooting experiment was carried out using a two phase procedure, root induction in media with high concentration of IBA, followed by transferring to growth regulators free medium for root development. No rooting was observed and further research is needed.

Cites

No record.

References

No record.

Cite

APA: Copy

SAADAT, Y.A., & ABBASI, A.. (2018). Effects of different growth regulators, gelling agents and carbon sources on in vitro growth of Berberis vulgaris var. asperma. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 26(1 (51) ), 121-131. SID. https://sid.ir/paper/104396/en

Vancouver: Copy

SAADAT Y.A., ABBASI A.. Effects of different growth regulators, gelling agents and carbon sources on in vitro growth of Berberis vulgaris var. asperma. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2018;26(1 (51) ):121-131. Available from: https://sid.ir/paper/104396/en

IEEE: Copy

Y.A. SAADAT, and A. ABBASI, “Effects of different growth regulators, gelling agents and carbon sources on in vitro growth of Berberis vulgaris var. asperma,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 26, no. 1 (51) , pp. 121–131, 2018, [Online]. Available: https://sid.ir/paper/104396/en

Related Journal Papers