EFFECTS OF PLANT GROWTH REGULATORS AND CULTURE MEDIA ON SHOOT PROLIFERATION AND REDUCTION OF VITRIFICATION OF PRUNUS MAHALEB L. VIA IN VITRO CULTURE

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GOODARZI GH.R.; PAYAM NOUR V.; JAFARI M.; ALIARAB A.

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Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2016 | Volume:24 | Issue:1 Page(s): 1-12

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Title

EFFECTS OF PLANT GROWTH REGULATORS AND CULTURE MEDIA ON SHOOT PROLIFERATION AND REDUCTION OF VITRIFICATION OF PRUNUS MAHALEB L. VIA IN VITRO CULTURE

Author(s)

GOODARZI GH.R. | PAYAM NOUR V. | JAFARI M. | ALIARAB A. | Issue Writer Certificate

Keywords

PLANT GROWTH REGULATORSQ1

PROLIFERATIONQ1

PHLOROGLUCINOLQ3

SINGLE NODE CULTUREQ1

VITRIFICATIONQ1

Abstract

Effects of different concentrations of PLANT GROWTH REGULATORS and culture media on shoot PROLIFERATION and VITRIFICATION of Prunus mahaleb L. via in vitro culture was studied using single node explants in two separate experiments. Shoots from single node explants were grown on 26 treatments (first experiment) of MS medium supplemented with different concentrations of cytokinins (BAP, TDZ, KIN, 2iP, and Zeatin) in combination with NAA and on 16 treatments (second experiment) of MS, 1.2 MS, WPM and DKW media supplemented with different combinations of CaCl2 and NH4NO3 and cytokinins of BAP, Zeatin, and PG. Experiments were based on completely randomized design with five replicates and each replication included 5 explants. Means comparison after 2 subcultures in the first experiment showed that the highest shoot length (3.14 cm) were obtained on 0.1 mg l-1 NAA in cytokinin -free medium, the highest number of shoot (14.67 shoot numbers per explants) on 0.2 mg l-1 Zeatin plus 0.1 mg l-1 NAA, and the lowest VITRIFICATION on 0.1 mg l-1 KIN, 0.05 and 0.1 mg l-1 Zeatin plus 0.1 mg l-1 NAA, respectively. In the second experiment, the highest shoot length (2.88 cm) were observed on DKW medium with 0.2 mg l-1 Zeatin plus 40 mg l-1 PG and the highest number of shoot (19 shoot number per explants) on MS medium with 880 mg l-1 CaCl2 and 825 mg l-1 NH4NO3. The present research by accessing to the highest PROLIFERATION (19 shoot numbers per explants) and decreasing VITRIFICATION of Prunus mahaleb L. provided a possibility of doing other research projects for protocol commercialization.

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APA: Copy

GOODARZI, GH.R., PAYAM NOUR, V., JAFARI, M., & ALIARAB, A.. (2016). EFFECTS OF PLANT GROWTH REGULATORS AND CULTURE MEDIA ON SHOOT PROLIFERATION AND REDUCTION OF VITRIFICATION OF PRUNUS MAHALEB L. VIA IN VITRO CULTURE. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 24(1), 1-12. SID. https://sid.ir/paper/104562/en

Vancouver: Copy

GOODARZI GH.R., PAYAM NOUR V., JAFARI M., ALIARAB A.. EFFECTS OF PLANT GROWTH REGULATORS AND CULTURE MEDIA ON SHOOT PROLIFERATION AND REDUCTION OF VITRIFICATION OF PRUNUS MAHALEB L. VIA IN VITRO CULTURE. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2016;24(1):1-12. Available from: https://sid.ir/paper/104562/en

IEEE: Copy

GH.R. GOODARZI, V. PAYAM NOUR, M. JAFARI, and A. ALIARAB, “EFFECTS OF PLANT GROWTH REGULATORS AND CULTURE MEDIA ON SHOOT PROLIFERATION AND REDUCTION OF VITRIFICATION OF PRUNUS MAHALEB L. VIA IN VITRO CULTURE,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 24, no. 1, pp. 1–12, 2016, [Online]. Available: https://sid.ir/paper/104562/en

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