Optimizing the regeneration and rooting of Iranian native shallot (Allium stipitatum) via in vitro

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Hajiheidar a.; TOHIDFAR M.; MIRI S.M.; ZAREKARIZI A.R.; GHADERMAZI S.; Samiee Kh.

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Journal: IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS Year:2017 | Volume:33 | Issue:2 (82) Page(s): 244-255

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Title

Optimizing the regeneration and rooting of Iranian native shallot (Allium stipitatum) via in vitro

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Keywords

Allium stipitatimQ1

regenerationQ2

rootingQ2

activated charcoalQ2

Abstract

This research was aimed to achieve the optimal protocol for micropropagation of shallot (Allium stipitatum) via basal disc. For regeneration, the basal disc explants were cultured on Murashig & Skoog (MS) medium containing different concentrations of Benzyladenine (BA) (0, 1 & 2 mgl-1) and Naphthalene acetic acid (NAA) (0, 0. 5 & 1 mgl-1). The regenerated bulblets were rooted on MS and ½ MS medium supplemented with different concentrations of BA, NAA, Indole-3-acetic acid (IAA) and activated charcoal (AC). The rooted plantlets were successfully acclimatized under ex vitro conditions. The results indicated that the maximum number of bulblet (12. 66) was observed on medium containing 2 mg. l-1 BA and 1 mg. l-1 NAA. In addition, the highest root number (1. 77) and length (2. 55 cm) were observed on 1 mg. l-1 IAA and 1 g. l-1 activated charcoal and the survival rate was 100% in pots filled with a coco peat: perlite: peat moss (1: 2: 1 v/v) mixture. This research could be a suitable method for micropropagation of this endangered plant. Hence, the rapid micropropagation of shallot is compulsory because of its various applications and extinction.

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APA: Copy

Hajiheidar, a., TOHIDFAR, M., MIRI, S.M., ZAREKARIZI, A.R., GHADERMAZI, S., & Samiee, Kh.. (2017). Optimizing the regeneration and rooting of Iranian native shallot (Allium stipitatum) via in vitro. IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS, 33(2 (82) ), 244-255. SID. https://sid.ir/paper/104951/en

Vancouver: Copy

Hajiheidar a., TOHIDFAR M., MIRI S.M., ZAREKARIZI A.R., GHADERMAZI S., Samiee Kh.. Optimizing the regeneration and rooting of Iranian native shallot (Allium stipitatum) via in vitro. IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS[Internet]. 2017;33(2 (82) ):244-255. Available from: https://sid.ir/paper/104951/en

IEEE: Copy

a. Hajiheidar, M. TOHIDFAR, S.M. MIRI, A.R. ZAREKARIZI, S. GHADERMAZI, and Kh. Samiee, “Optimizing the regeneration and rooting of Iranian native shallot (Allium stipitatum) via in vitro,” IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS, vol. 33, no. 2 (82) , pp. 244–255, 2017, [Online]. Available: https://sid.ir/paper/104951/en

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