Effects of methyl jasmonate and salicylic acid on production of betulin and betulinic acid in cell suspension of birch (Betula pendula Roth. )

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

JAFARI HAJATI R.; PAYAMNOOR V.; GHASEMI BEZDI K.; AHMADIAN CHASHMI N.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS Year:2017 | Volume:32 | Issue:6 (80) Page(s): 1037-1047

Download Full-Text

Persian Verion

View:

542

Download:

Cites:

Information Journal Paper

Title

Effects of methyl jasmonate and salicylic acid on production of betulin and betulinic acid in cell suspension of birch (Betula pendula Roth. )

Author(s)

JAFARI HAJATI R. | PAYAMNOOR V. | GHASEMI BEZDI K. | AHMADIAN CHASHMI N. | Issue Writer Certificate

Keywords

BetulinQ1

betulinic acidQ1

elicitorQ1

HPLCQ2

Abstract

This study aimed to enhance the production of Betulin and Betulinic acid using suspension cultures of birch (Betula pendula Roth) and elicitation of the cell cultures by methyl jasmonate (MeJA) and salicylic acid (SA). To do this, at the first step, the cell growth curve was investigated in a 16-day period. Then, two elicitors, namely, MeJA (at final concentration of 0, 50, 100, 150 and 200 μ M) and SA (at final concentration of 0, 100, 200, 300 and 400 μ M) were separately supplemented to 8-day-old cell cultures and the cells were harvested 1, 2, 3, 5 and 7 days after elicitations. Fresh weight (FW), dry weight (DW) and cell viability were measured. In addition, Betulin and Betulinic acid content were analyzed using HPLC. The results showed the significant effects of different concentrations of SA and MeJA on metabolites content and FW and DW. Maximum amount of Betulin was observed about 4-fold (2. 5 mg g-1 DW) higher than the control treatment by addition of 100 μ M SA, two days after elicitation. Moreover, Betulinic acid content was enhanced about 5 mg g-1 DW, 4. 5-fold compared to control, one day after addition of 200 μ M SA. Furthermore, the high accumulation of Betulin (2. 3 mg g-1 DW) was obtained in the elicited cell by 50 μ M MeJA, seven days after elicitation. Also, the maximum amount of Betulinic acid, about 3 mg g-1 DW, was observed in the cells elicited by 100 μ M MeJA, two days after elicitation. Overall, the effect of SA on the production of Betulin and Betulinic acid was significantly more than the effect of MeJA.

Cites

No record.

References

No record.

Cite

APA: Copy

JAFARI HAJATI, R., PAYAMNOOR, V., GHASEMI BEZDI, K., & AHMADIAN CHASHMI, N.. (2017). Effects of methyl jasmonate and salicylic acid on production of betulin and betulinic acid in cell suspension of birch (Betula pendula Roth. ). IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS, 32(6 (80) ), 1037-1047. SID. https://sid.ir/paper/105488/en

Vancouver: Copy

JAFARI HAJATI R., PAYAMNOOR V., GHASEMI BEZDI K., AHMADIAN CHASHMI N.. Effects of methyl jasmonate and salicylic acid on production of betulin and betulinic acid in cell suspension of birch (Betula pendula Roth. ). IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS[Internet]. 2017;32(6 (80) ):1037-1047. Available from: https://sid.ir/paper/105488/en

IEEE: Copy

R. JAFARI HAJATI, V. PAYAMNOOR, K. GHASEMI BEZDI, and N. AHMADIAN CHASHMI, “Effects of methyl jasmonate and salicylic acid on production of betulin and betulinic acid in cell suspension of birch (Betula pendula Roth. ),” IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS, vol. 32, no. 6 (80) , pp. 1037–1047, 2017, [Online]. Available: https://sid.ir/paper/105488/en

Related Journal Papers