Improvement of friable callus induction of Crocus sativus L. and establishment of a cell suspension culture system with high biomass

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Amini Somaye; Ziaratnia Seyed Mahdi; Rajabzadeh Ghadir

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Journal Paper

Paper Information

Journal: JOURNAL OF HORTICULTURE AND POSTHARVEST RESEARCH Year:2023 | Volume:6 | Issue:4 Page(s): 397-408

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Information Journal Paper

Title

Improvement of friable callus induction of Crocus sativus L. and establishment of a cell suspension culture system with high biomass

Author(s)

Amini Somaye | Ziaratnia Seyed Mahdi | Rajabzadeh Ghadir | Issue Writer Certificate

Keywords

Callogenesis

Glutamine

MS medium

Saffron

Solid medium

Abstract

Purpose: This study aims to explore the potential of in vitro culture as a method for scaling up the production of Saffron based medicinal compounds, the most expensive spice renowned. Emphasis is placed on the critical role of friable callus (FC) formation as a prerequisite for successful suspension culture. Research method: The research primarily investigates FC formation, focusing on the impact of varying strengths of Murashige and Skoog (MS) medium as well as combinations of NAA or 2,4-D and BA or Kin on compact callus. Subsequently, the study involves supplementing the MS medium with different concentrations of 2,4-D, kin, zeatin, Glutamine, sucrose, and nitrogen to establish a cell suspension culture. Findings: The highest FC yield was achieved on a Solid medium containing 2,4-D (1 mg l-1)+Kin (0.2 mg l-1), resulting in a fresh weight (FW) of 0.413 g. Furthermore, MS combined with 2,4-D (1 mg l-1)+Kin (0.2 mg l-1)+Glutamine (10 mg l-1), as well as MS+2,4-D (0.5 mg l-1)+zeatin (0.3 mg l-1)+Glutamine (10 mg l-1), demonstrated the highest FW under suspension conditions. The study also identified that 30 g l-1 sucrose and 30 µM were optimal for inducing maximum FW. Research limitations: Cell biomass is influenced by several factors that should to be optimized. Originality/Value: This research concludes that a cell suspension system holds promise for rapidly generating sufficient cell biomass to produce valuable secondary metabolites within a limited timeframe and space. Notably, the system successfully increased biomass from 0.2 to 1.2 g, underscoring its potential for efficient Saffron-based product development.

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