Investigation of coliforms and antibiotic resistant Escherichia coli occurrence in the Caspian Sea coast-Guilan province

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

FARHANGI MOHAMMAD BAGHER; Ghorbanzadeh Nasrin

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF WATER AND SOIL CONSERVATION Year:2021 | Volume:28 | Issue:3 Page(s): 173-190

Download Full-Text

Persian Verion

View:

Download:

Cites:

Information Journal Paper

Title

Investigation of coliforms and antibiotic resistant Escherichia coli occurrence in the Caspian Sea coast-Guilan province

Author(s)

FARHANGI MOHAMMAD BAGHER | Ghorbanzadeh Nasrin | Issue Writer Certificate

Keywords

Ceftriaxone

Cephalexin

Chamkhaleh

Roudsar

ZOI

Abstract

Background and Objectives: In recent years, many coastal areas have been exposed to pollutant threats. The occurrence of pathogenic bacteria in recreational beaches has made difficulties in these areas. Resistance of these bacteria to antibiotics has also worsened the situation. Therefore, this study aimed to investigate the occurrence of coliforms and antibiotic resistant Escherichia coli, as an indicator bacterium, in the swash zone of Caspian Sea, Guilan province. Materials and Methods: Nine popular coastal areas in Guilan province including Rudsar, Chamkhaleh, Aminabad, Jafroud, Sahele-Ghou (Anzali), Rezvanshahr, Gisum, Sahele-Zomorrod (Haviq), and Astara were selected for this study. The sand beaches were sampled in three replications from 0-20 cm depth in the swash zone. The coliform number was counted on the EMB medium. pH and EC were also measured in 2: 1 sand: distilled water suspension. Then, E. coli as an indicator bacteria was isolated and its resistance to antibiotics was determined by disk diffusion method on Mueller-Hinton agar medium. Broad-spectrum antibiotics including cephalexin, gentamicin, doxycycline, ceftriaxone, ciprofloxacin, and trimethoprim were tested. Data for pH, EC and coliform number were analyzed in a completely randomized design and for the zone of inhibition (ZOI) test, a factorial arrangement experiment in a completely randomized design was adopted. Also, mean comparisons were done by Tukey test (P<0. 05). Results: In all sampling areas, the pH was alkaline and its highest value was obtained in the Jafroud beach sand. The highest EC value was obtained in Aminabad beach sand which was significantly different from EC values in other sampled sites (P<0. 05). The highest number of coliforms was obtained in Sahele-Zomorrod sand beach (5. 8 Log CFU g-1). The effect of sampling site and antibiotic interactions on the ZOI of E. coli showed that in three sampling sites including Astara, Gisum and Rezvanshahr the isolated E. coli was resistant to none of the tested antibiotics. In the other six sites except Sahele-Ghou (Anzali), it showed resistance to cephalexin. The isolated E. coli showed resistance only to cephalexin in Jafroud. While it also showed resistance to ceftriaxone in Rudsar, Chamkhaleh (eastern beaches), Sahele-Ghou, and Sahele-Zomorrod. Conclusion: Overall, the beach sand of the western coastal areas had a lower pH and EC values and higher total coliform numbers compared to the eastern and central coastal areas. In all studied places, the coliform number was above the standard level for recreational sites. In eastern coastal areas,Chamkhaleh and Roudsar, isolated E. coli showed resistance to both cephalexin and ceftriaxone antibiotics. Thus, the management of microbial contamination especially those that are resistant to antibiotics needs more attention in the eastern coastal areas in Guilan province.

Multimedia

No record.

Cites

No record.

References

No record.

Cite

APA: Copy

FARHANGI, MOHAMMAD BAGHER, & Ghorbanzadeh, Nasrin. (2021). Investigation of coliforms and antibiotic resistant Escherichia coli occurrence in the Caspian Sea coast-Guilan province. JOURNAL OF WATER AND SOIL CONSERVATION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES), 28(3 ), 173-190. SID. https://sid.ir/paper/1137812/en

Vancouver: Copy

FARHANGI MOHAMMAD BAGHER, Ghorbanzadeh Nasrin. Investigation of coliforms and antibiotic resistant Escherichia coli occurrence in the Caspian Sea coast-Guilan province. JOURNAL OF WATER AND SOIL CONSERVATION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES)[Internet]. 2021;28(3 ):173-190. Available from: https://sid.ir/paper/1137812/en

IEEE: Copy

MOHAMMAD BAGHER FARHANGI, and Nasrin Ghorbanzadeh, “Investigation of coliforms and antibiotic resistant Escherichia coli occurrence in the Caspian Sea coast-Guilan province,” JOURNAL OF WATER AND SOIL CONSERVATION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES), vol. 28, no. 3 , pp. 173–190, 2021, [Online]. Available: https://sid.ir/paper/1137812/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops