Research Article: Effect of enzyme type, enzyme to substrate ratio, temperature and hydrolysis time on the yield and antioxidant effect of Saurida tumbil protein hydrolysate

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Bahram S.; Khezri M.; Javadian S.R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Fisheries Sciences Year:621 | Volume: | Issue: Page(s): 1397-1416

Download Full-Text

View:

Download:

Cites:

Information Journal Paper

Title

Research Article: Effect of enzyme type, enzyme to substrate ratio, temperature and hydrolysis time on the yield and antioxidant effect of Saurida tumbil protein hydrolysate

Author(s)

Bahram S. | Khezri M. | Javadian S.R. | Issue Writer Certificate

Keywords

AlcalaseQ4

PapainQ4

Fish Protein HydrolysateQ4

Saurida tumbilQ4

Antioxidative peptidesQ4

Abstract

It has become a hot topic to make optimal use of fisheries products and make them value added as well as find natural food substances which can effectively inhibit oxidative stress and improve human health. Given this, Saurida tumbil muscle was hydrolyzed by Alcalase and Papain at two concentrations of 2 and 4%, and at two hydrolysis times of 90 and 180 min and their antioxidant properties were compared. Antioxidant activity of the protein hydrolysates were investigated by ferrous chelating and ferric reducing, 2, 2-Diphenyl-1-picrylhydrazyl (DPPH), 2, 2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl free radical scavenging activity tests. Samples hydrolyzed by Papain showed higher protein recovery than those hydrolyzed by Alcalase (59. 9% versus 20. 06%). The protein hydrolysate prepared by Papain enzyme showed significantly higher activity in removal of DPPH (IC50=2. 44 mg/mL) and hydroxyl (IC50=3. 93 mg/mL) free radicals than those prepared by Alcalase enzyme (DPPH IC50=2. 80 mg/mL, hydroxyl IC50=8. 59 mg/mL). ABTS radical scavenging activity did not show any significant difference between hydrolyzed samples with Papain or Alcalase enzymes. Hydrolyzed samples with Alcalase enzyme showed higher ferrous chelating activity (IC50=5. 33 mg/mL) and ferric reducing activity (optical absorption=0. 13±00 at 700 nm) than those hydrolysate prepared by Papain enzyme. Generally, the type of enzyme, its concentration and the time of hydrolysis affected the yield and antioxidant properties of Saurida tumbil muscle protein hydrolysates and hydrolysis with Papain at concentration of 4% for 180 min provided the best properties.

Cites

No record.

References

No record.

Cite

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops