Comparative gut Microbiome Evaluation of two Distinct Organisms: A Critical Review

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Paritala Venu

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Scientific Information Database

ISSN: 2588-4824

Journal Paper

Paper Information

Journal: Journal of Agricultural Sciences and Engineering Year:621 | Volume: | Issue: Page(s): 117-126

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Information Journal Paper

Title

Comparative gut Microbiome Evaluation of two Distinct Organisms: A Critical Review

Author(s)

Paritala Venu | Issue Writer Certificate

Keywords

MG-RASTQ4

QIIMEQ4

Metagenomic AnalysisQ4

Next-Generation Sequencing TechniquesQ4

Taxonomy AnalysisQ4

Diversity AnalysisQ4

Abstract

Background: The investigation of microbial community composition has been significantly influenced by technological innovations in the next-generation sequencing-based techniques. These techniques depend on 16S rRNA, in particular, have been frequently used to analyse the whole collection of bacteria present in a specific environment. As a response, a plethora of specific bioinformatic pipelines has been constructed for handling this data. Methods and Analysis: Meta Genome Rapid Annotation Using Subsystem Technology (MG-RAST) and Quantitative Insights into Microbial Ecology (QIIME) are two publicly accessible Metagenomic Analysis tools utilized in several investigations. Here, we present a comparative study of the human vs. chimpanzee dataset using QIIME and MG-RAST to assess their accuracy in taxonomy and Diversity Analysis. Discussion: Here, the taxonomic assignment was more accurate using QIIME, which assigned a considerably greater number of reads at the family level. As a consequence, QIIME produced a more accurate sequence file, which enhanced the Diversity Analysis outcome. Finally, while installing QIIME requires informatics expertise, it provides a wide range of metrics that are relevant for downstream applications and, more importantly, it is not dependent on server delays.

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