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Information Journal Paper

Title

Quantitative Modeling of Arsenic Removal from Aqueous Solution by CTAB-modified Zeolite Using Central Composite Design

Pages

  87-99

Abstract

 Mulberry is a single-stemmed tree, growing in a variety of climates. Mulberry leaves are the main source of food for silkworm, which plays a key role in silk industry. In recent decades, since the use of chemical fertilizers has caused many environmental problems, the application of organic and bio-fertilizers has increased significantly. The current study aimed to investigate the impact of growth promoting bacteria and municipal waste on enzyme changes in mulberry leaves as well as soil phosphatase enzyme. For this research, 27 mulberry seedlings were collected from Iran Silk Research Centre. This research was conducted as a completely randomized design with three replications in the research greenhouse of Guilan University. In this study, the treatments used included two levels of municipal waste (two and four percent), two levels of Pseudomonas sp. inocula (106 and 5×106 cells per liter) and control sample. Catalase, polyphenol oxidase and superoxide dismutase, chlorophyll a and b in mulberry leaves were measured and also soil phosphatase was determined. The results indicated that the application of bacteria inocula led to the increment in the amount of soil phosphatase enzyme. The results also showed that the amount of superoxide dismutase enzyme were increased from 77.2 in the control treatment to 276 μmol/g of fresh leaf tissue in the higher level of compost and bacteria treatment (second level). Furthermore, the highest amount of this enzyme was equal to 0.16 (μmol · min−1 · g−1 wet weight), which was observed in the higher level of Pseudomonas sp. inoculum (5×106) along with the higher level of organic fertilizer (4%). In general, the results demonstrated that a combination of organic fertilizer and beneficial bacteria inocula can be used instead of chemical fertilizers to provide the elements required by the mulberry plant.

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