STUDY OF POLYMORPHISMS IN THE INTRONS 1 AND 4 CHICKEN GROWTH HORMONE GENE IN THE NATIVE FOWLS OF ISFAHAN AND MAZANDARAN

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JAFARI AZAM; PAKDEL A.; ESMAIL KHANIAN S.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2009 | Volume:4 | Issue:3 Page(s): 37-43

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Title

STUDY OF POLYMORPHISMS IN THE INTRONS 1 AND 4 CHICKEN GROWTH HORMONE GENE IN THE NATIVE FOWLS OF ISFAHAN AND MAZANDARAN

Author(s)

JAFARI AZAM | PAKDEL A. | ESMAIL KHANIAN S. | Issue Writer Certificate

Keywords

GROWTH HORMONEQ3

POLYMORPHISMQ2

NATIVE FOWLSQ2

PCR-RFLPQ3

Abstract

The chicken GROWTH HORMONE (cGH) is a polypeptide hormone which is involved in a wide variety of physiological functions. To investigate the GROWTH HORMONE gene POLYMORPHISM, 217 samples (129 samples of Isfahan and 88 samples of mazandaran) for intron 1 and 134 samples (91 samples of Isfahan and 43 samples of mazandaran) for intron 4 were analyzed. The genomic DNA was extracted using salting out technique. The quantity and the quality of the extracted DNA was examined using spectrophotometery and then analyzed on a 0.8% agarose gel electrophoresis. A fragment with the size of 776 bp from the intron 1 and a fragment with the size of 1170 bp from the intron 4 were amplified using two pair of specific primers and polymerase chain reaction. The PCR products digested with MspI restriction enzyme and then it analyzed on %2.5 agarose gel. The allelic frequency of intron 1 locus for M, N and O alleles in Isfahan NATIVE FOWLS was 0.60, 0.21 & 0.19 respectively and in Mazandaran populations was 0.28, 0.05, 0.67 respectively. The allelic frequency of intron 4 for A, B and C alleles in Isfahan NATIVE FOWLS populations were 0.28, 0.31 & 0.41 respectively and in Mazandaran population were 0.37, 0.24 & 0.37 respectively. Also D and E alleles were observed in Mazandaran population solely with 0.01 frequency for each allele. The result of current study indicated that the introns 1 and 4 of cGH is relatively highly polymorphic in Isfahan and Mazandaran NATIVE FOWLS.

Cites

GENETIC DIVERSITY OF INTERON 4 OF GROWTH HORMONE GENE IN WEST AZERBAIJAN NATIVE CHICKEN USING PCR-RFLP TECHNIQUE

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APA: Copy

JAFARI, AZAM, PAKDEL, A., & ESMAIL KHANIAN, S.. (2009). STUDY OF POLYMORPHISMS IN THE INTRONS 1 AND 4 CHICKEN GROWTH HORMONE GENE IN THE NATIVE FOWLS OF ISFAHAN AND MAZANDARAN. MODERN GENETICS JOURNAL (MGJ), 4(3), 37-43. SID. https://sid.ir/paper/116577/en

Vancouver: Copy

JAFARI AZAM, PAKDEL A., ESMAIL KHANIAN S.. STUDY OF POLYMORPHISMS IN THE INTRONS 1 AND 4 CHICKEN GROWTH HORMONE GENE IN THE NATIVE FOWLS OF ISFAHAN AND MAZANDARAN. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2009;4(3):37-43. Available from: https://sid.ir/paper/116577/en

IEEE: Copy

AZAM JAFARI, A. PAKDEL, and S. ESMAIL KHANIAN, “STUDY OF POLYMORPHISMS IN THE INTRONS 1 AND 4 CHICKEN GROWTH HORMONE GENE IN THE NATIVE FOWLS OF ISFAHAN AND MAZANDARAN,” MODERN GENETICS JOURNAL (MGJ), vol. 4, no. 3, pp. 37–43, 2009, [Online]. Available: https://sid.ir/paper/116577/en

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