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Information Journal Paper

Title

POLYMORPHISM OF INTERON I OF CHICKEN GROWTH HORMONE GENE IN WEST AZERBAIJAN NATIVE CHICKEN USING PCR-RFLP

Pages

  21-30

Abstract

 In order to study of the POLYMORPHISM of Interon 1 of chicken GROWTH HORMONE gene (cGH) in WEST AZERBAIJAN NATIVE CHICKEN, blood samples were collected from 98 birds. Genomic DNA was extracted and a fragment of 776 bp in size was amplified using polymerase chain reaction. The amplified fragments were subjected to restriction digestion with Msp I endonuclease and the resultant digested products were run on 2% agarose gel. The results revealed the existence of three alleles A1, A2 and A3 for the examined locus with frequencies of 31.12, 17.86 and 51.02 percent, respectively. From six different genotypic variants, the genotypes of A1A3 (31.63%) and A2A2 (1.02%) had the highest and lowest frequencies, respectively. The expected heterozygosity and effective number of alleles were 0.61 and 2.57, respectively. Chi- square and G- tests showed that this population is in Hardy- Weinberg equilibrium. It can be concluded that Interon 1 of the cGH gene of WEST AZERBAIJAN NATIVE CHICKEN has high genetic variation which can be considered for future breeding programs.

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    APA: Copy

    KHAKPOUR, K., MARDANI, K., & HASHEMI, A.. (2012). POLYMORPHISM OF INTERON I OF CHICKEN GROWTH HORMONE GENE IN WEST AZERBAIJAN NATIVE CHICKEN USING PCR-RFLP. ANIMAL SCIENCE RESEARCHES (FACULTY OF AGRICULTURE, UNIVERSITY OF TABRIZ), 21(3), 21-30. SID. https://sid.ir/paper/147540/en

    Vancouver: Copy

    KHAKPOUR K., MARDANI K., HASHEMI A.. POLYMORPHISM OF INTERON I OF CHICKEN GROWTH HORMONE GENE IN WEST AZERBAIJAN NATIVE CHICKEN USING PCR-RFLP. ANIMAL SCIENCE RESEARCHES (FACULTY OF AGRICULTURE, UNIVERSITY OF TABRIZ)[Internet]. 2012;21(3):21-30. Available from: https://sid.ir/paper/147540/en

    IEEE: Copy

    K. KHAKPOUR, K. MARDANI, and A. HASHEMI, “POLYMORPHISM OF INTERON I OF CHICKEN GROWTH HORMONE GENE IN WEST AZERBAIJAN NATIVE CHICKEN USING PCR-RFLP,” ANIMAL SCIENCE RESEARCHES (FACULTY OF AGRICULTURE, UNIVERSITY OF TABRIZ), vol. 21, no. 3, pp. 21–30, 2012, [Online]. Available: https://sid.ir/paper/147540/en

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