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Information Journal Paper

Title

EFFECT OF SALT STRESS ON EXPRESSION OF KEY GENES IN FRUCTAN METABOLISM IN WHEAT AT ANTHESIS

Pages

  229-238

Abstract

FRUCTANs as the main WHEAT stem reserves are accumulated during SALT STRESS and their remobilization into grains can be significant to reduce severe yield loss. To study the effect of salinity on WHEAT FRUCTAN metabolism at molecular level, expression of the key genes including sucrose-sucrose fructosyl transferase (1-SST) and sucrose- FRUCTAN fructosyl transfarease (6-SFT) involved in FRUCTAN biosynthesis, fructosyl exohydrolase (1-FEH) and vacuolar invertase (IVR) involved in FRUCTAN and sucrose degradation, respectively, and sucrose transporter (SUT1) was analyzed in Bam as a salt-tolerant and Ghods as a salt-sensitive WHEAT cultivars. Control and salt treatments were applied by irrigation water with EC of 0.5 and 12 dSm-1, respectively from seedling stage in the greenhouse with three replicates using completely randomized design. GENE EXPRESSION analysis was carried out at anthesis on leaf and stem by REAL TIME PCR and stem FRUCTAN measurement was done at five points with 7-day intervals during seed filling period. The results showed the up-regulation of stem 1-SST and 6-SFT and the increase of FRUCTAN content in Bam and down-regulation of these genes and decrease of FRUCTAN content in Ghods under salinity. In leaf tissues of both cultivars, 1-SST, 1-FEH and SUT1 up-regulated under SALT STRESS. Based on the obtained results, the most remarkable difference of FRUCTAN metabolism involved GENE EXPRESSION under SALT STRESS between Bam and Ghods at anthesis was related to stem 6-SFT gene. The results showed that the up-regulation of 6-SFT, the higher FRUCTAN production and having greater capacity of stem reserves in Bam correlates with its higher grain yield under SALT STRESS.

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  • Cite

    APA: Copy

    SHARBATKHARI, M., SHOBBAR, Z.S., SARABADANI, R., & ALAVI, S.. (2014). EFFECT OF SALT STRESS ON EXPRESSION OF KEY GENES IN FRUCTAN METABOLISM IN WHEAT AT ANTHESIS. MODERN GENETICS JOURNAL (MGJ), 9(2), 229-238. SID. https://sid.ir/paper/116626/en

    Vancouver: Copy

    SHARBATKHARI M., SHOBBAR Z.S., SARABADANI R., ALAVI S.. EFFECT OF SALT STRESS ON EXPRESSION OF KEY GENES IN FRUCTAN METABOLISM IN WHEAT AT ANTHESIS. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2014;9(2):229-238. Available from: https://sid.ir/paper/116626/en

    IEEE: Copy

    M. SHARBATKHARI, Z.S. SHOBBAR, R. SARABADANI, and S. ALAVI, “EFFECT OF SALT STRESS ON EXPRESSION OF KEY GENES IN FRUCTAN METABOLISM IN WHEAT AT ANTHESIS,” MODERN GENETICS JOURNAL (MGJ), vol. 9, no. 2, pp. 229–238, 2014, [Online]. Available: https://sid.ir/paper/116626/en

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