INVESTIGATION OF GENETIC RELATIVE OF TRITIPYRUM (EBEB), TRITICALE (RR) AND WHEAT (ABD) GENOMES USING PCR BASED MOLECULAR MARKERS

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ALLAHDO M.; SIAHSAR B.A.; SHAHSAVAND HASSANI H.; NAROUI RAD M.; KAZEMIPOOR A.; IMAMJOMEH A.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2009 | Volume:3 | Issue:4 Page(s): 75-83

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Title

INVESTIGATION OF GENETIC RELATIVE OF TRITIPYRUM (EBEB), TRITICALE (RR) AND WHEAT (ABD) GENOMES USING PCR BASED MOLECULAR MARKERS

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Keywords

GENETIC RELATIVEQ3

TRITIPYRUMQ3

TRITICALEQ3

WHEATQ2

TH. BESSARABICUMQ3

Abstract

In order to determine GENETIC RELATIVE between TRITICALE, TRITIPYRUM and WHEAT genomes, genomic DNA of Thinopyrum bessarabicum (EbEb), TRITICALE premising lines (AABBRR), TRITIPYRUM primary lines (AABBEbEb), Chinese spring and breeding cultivars of WHEAT (AABBDD) were amplified using 25 semi random and 30 random primers. Bands that present in the TH. BESSARABICUM, TRITICALE premising lines and TRITIPYRUM lines, but absent in the breeding and Chinese spring cultivars of WHEAT, assumed as shared bands between TRITICALE (RR) and TRITIPYRUM (EbEb) genomes. Jaccard coefficient of similarity used for determination of GENETIC RELATIVE of TRITICALE (RR) and TRITIPYRUM (EbEb) with WHEAT genomes. ET34 and ET37 of semi random and OPM06 of random primers could be amplified shared bands between TRITICALE (RR) and TRITIPYRUM (EbEb) genomes. From 238 bands that amplified by random and semirandom primers, 5 bands (2.2%) were shared between TRITICALE (RR) and TRITIPYRUM (EbEb) and 16.8% were shared between TRITIPYRUM (EbEb) and WHEAT genomes. 4.02% of RAPD markers, were specified to TRITIPYRUM (EbEb) genome. Average genetic similarity between TRITICALE premising lines, TRITIPYRUM primary lines, and TH. BESSARABICUM were 0.179, between TRITIPYRUM primary lines and WHEAT breeding cultivars were 0.194 and between TRITICALE premising lines and WHEAT breeding cultivars were 0.161. This study confirmed high homology of TRITIPYRUM (EbEb) with WHEAT, relatively homology of TRITICALE (RR) and TRITIPYRUM (EbEb) genomes and usefulness of random and semi random primers in detecting chromatin/DNA fragment of intergeneric hybrids with durum WHEAT.

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APA: Copy

ALLAHDO, M., SIAHSAR, B.A., SHAHSAVAND HASSANI, H., NAROUI RAD, M., KAZEMIPOOR, A., & IMAMJOMEH, A.. (2009). INVESTIGATION OF GENETIC RELATIVE OF TRITIPYRUM (EBEB), TRITICALE (RR) AND WHEAT (ABD) GENOMES USING PCR BASED MOLECULAR MARKERS. MODERN GENETICS JOURNAL (MGJ), 3(4), 75-83. SID. https://sid.ir/paper/116640/en

Vancouver: Copy

ALLAHDO M., SIAHSAR B.A., SHAHSAVAND HASSANI H., NAROUI RAD M., KAZEMIPOOR A., IMAMJOMEH A.. INVESTIGATION OF GENETIC RELATIVE OF TRITIPYRUM (EBEB), TRITICALE (RR) AND WHEAT (ABD) GENOMES USING PCR BASED MOLECULAR MARKERS. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2009;3(4):75-83. Available from: https://sid.ir/paper/116640/en

IEEE: Copy

M. ALLAHDO, B.A. SIAHSAR, H. SHAHSAVAND HASSANI, M. NAROUI RAD, A. KAZEMIPOOR, and A. IMAMJOMEH, “INVESTIGATION OF GENETIC RELATIVE OF TRITIPYRUM (EBEB), TRITICALE (RR) AND WHEAT (ABD) GENOMES USING PCR BASED MOLECULAR MARKERS,” MODERN GENETICS JOURNAL (MGJ), vol. 3, no. 4, pp. 75–83, 2009, [Online]. Available: https://sid.ir/paper/116640/en

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