ISOLATION OF HINDIII SATDNA IN PERSIAN STURGEON ACIPENSER PERSICUS

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NOWRUZFASHKHAMI M.R.; AZIZZADEH PORMEHR L.; MAHJOUBI F.; POURKAZEMI M.; KAZEMI B.; HASSANZADEH SABER M.; YARMOHHAMADI M.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2014 | Volume:9 | Issue:1 (36) Page(s): 121-126

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Title

ISOLATION OF HINDIII SATDNA IN PERSIAN STURGEON ACIPENSER PERSICUS

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Keywords

ACIPENSER PERSICUSQ2

CASPIAN SEAQ2

HIND III SATDNAQ2

PERSIAN STURGEONQ2

Abstract

The purpose of this study was to determine the presence or absence of a specific satellite DNA on the genus Acipenser (Hind III SatDNA) in PERSIAN STURGEON, ACIPENSER PERSICUS. Genomic DNA was extracted from caudal fin tissue of A. persicus specimen using the phenol-chloroform method to extract HIND III SATDNA. HIND III SATDNA amplified using the PCR technique and PCR product was evaluated by agarose gel electrophoresis after staining the gel with ethidium bromide. Obtained HIND III SATDNA fragment after extraction from agarose gel and purification, ligated to the plasmid PTZ/57R (Fermentas) and cloned in competent E. coli DH5α cells (Fermentas). Finally three clones containing the HIND III SATDNA fragment were sequenced. Sequencing of extracted HIND III SATDNA from genome of ACIPENSER PERSICUS showed that it contained 163bp which is registered in NCBI as FJ429174. Comparison HIND III SATDNA extracted from A. persicus in the present study with those extracted from Acipenser gueldenstaedtii, A. ruthenus, A. naccarii, A. brevirostrum, A. baeri, A. fulvescens, A. stellatus and A. sinensis showed 88% and with that from A. transmontanus showed 90% similarity. Presence HIND III SATDNA in A. persicusand high similarity between it and those from the mentioned sturgeons verify this fish belongs to Acipenser genus.

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APA: Copy

NOWRUZFASHKHAMI, M.R., AZIZZADEH PORMEHR, L., MAHJOUBI, F., POURKAZEMI, M., KAZEMI, B., HASSANZADEH SABER, M., & YARMOHHAMADI, M.. (2014). ISOLATION OF HINDIII SATDNA IN PERSIAN STURGEON ACIPENSER PERSICUS. MODERN GENETICS JOURNAL (MGJ), 9(1 (36)), 121-126. SID. https://sid.ir/paper/116674/en

Vancouver: Copy

NOWRUZFASHKHAMI M.R., AZIZZADEH PORMEHR L., MAHJOUBI F., POURKAZEMI M., KAZEMI B., HASSANZADEH SABER M., YARMOHHAMADI M.. ISOLATION OF HINDIII SATDNA IN PERSIAN STURGEON ACIPENSER PERSICUS. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2014;9(1 (36)):121-126. Available from: https://sid.ir/paper/116674/en

IEEE: Copy

M.R. NOWRUZFASHKHAMI, L. AZIZZADEH PORMEHR, F. MAHJOUBI, M. POURKAZEMI, B. KAZEMI, M. HASSANZADEH SABER, and M. YARMOHHAMADI, “ISOLATION OF HINDIII SATDNA IN PERSIAN STURGEON ACIPENSER PERSICUS,” MODERN GENETICS JOURNAL (MGJ), vol. 9, no. 1 (36), pp. 121–126, 2014, [Online]. Available: https://sid.ir/paper/116674/en

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