ANALYSIS OF SOME FACTORS ON GENE INJECTION EFFICIENCY TO MOUSE ZYGOTE

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DALIRI M.; KHAIRKHAHI OGHANI R.; ZOMORODIPOUR A.R.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2007 | Volume:2 | Issue:1 Page(s): 65-76

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Title

ANALYSIS OF SOME FACTORS ON GENE INJECTION EFFICIENCY TO MOUSE ZYGOTE

Author(s)

DALIRI M. | KHAIRKHAHI OGHANI R. | ZOMORODIPOUR A.R. | Issue Writer Certificate

Keywords

TRANSGENEQ3

EMBRYOQ2

EMBRYO MANIPULATIONQ3

MICROINJECTIONQ3

Abstract

Direct micro injection of recombinant gene is a regular technique for genetic alteration in mouse. In this study our aim was to optimize micro injection and also factors effecting the technique in mouse zygote. For this study, we prepared various sizes of micro injecting needles. Fertilized zygotes were harvested from super ovulated mouse treated with PMSG and hCG. Then, cDNA molecules of human growth hormone gene were injected into male pronuclus of mouse zygote and allowed to develop into 2 - 4 cell EMBRYO. Zona plucida were desolved, polymerase chain reaction were performed with specific primers for human growth hormone gene and also B-actin as a control, we also studied the response of different strains of mice to hormonal treatment and also effect of various diameter size of injecting needle on cell division of zygotes. Results indicated that type of needle, strains of mouse and also hormonal dose could affect the technique. However 90% of zygotes obtained from F1 (NMRI X BALB/c) mouse after DNA micro injection could proceed to 2 cell EMBRYO in DMEM/F12 culture media. Also, KSOM medium ceases arrest at 2 - cell stage of EMBRYO.

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APA: Copy

DALIRI, M., KHAIRKHAHI OGHANI, R., & ZOMORODIPOUR, A.R.. (2007). ANALYSIS OF SOME FACTORS ON GENE INJECTION EFFICIENCY TO MOUSE ZYGOTE. MODERN GENETICS JOURNAL (MGJ), 2(1), 65-76. SID. https://sid.ir/paper/116680/en

Vancouver: Copy

DALIRI M., KHAIRKHAHI OGHANI R., ZOMORODIPOUR A.R.. ANALYSIS OF SOME FACTORS ON GENE INJECTION EFFICIENCY TO MOUSE ZYGOTE. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2007;2(1):65-76. Available from: https://sid.ir/paper/116680/en

IEEE: Copy

M. DALIRI, R. KHAIRKHAHI OGHANI, and A.R. ZOMORODIPOUR, “ANALYSIS OF SOME FACTORS ON GENE INJECTION EFFICIENCY TO MOUSE ZYGOTE,” MODERN GENETICS JOURNAL (MGJ), vol. 2, no. 1, pp. 65–76, 2007, [Online]. Available: https://sid.ir/paper/116680/en

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