Investigating the effect of probiotics mixture (Lactobacillus casei, Lactobacillus rhamnosus and Lactobacillus heloticus) on small intestine tissue infected with Escherichia coli bacteria in male rats

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Hosseini Saba; Keshtmand Zahra; Mirnurollahi Seyyedeh Masumeh

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Journal Paper

Paper Information

Journal: JOURNAL OF APPLIED MICROBIOLOGY IN FOOD INDUSTRY Year:2023 | Volume:9 | Issue:2 Page(s): 79-87

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Title

Investigating the effect of probiotics mixture (Lactobacillus casei, Lactobacillus rhamnosus and Lactobacillus heloticus) on small intestine tissue infected with Escherichia coli bacteria in male rats

Author(s)

Hosseini Saba | Keshtmand Zahra | Mirnurollahi Seyyedeh Masumeh | Issue Writer Certificate

Keywords

Probiotic

Escherichia coli

Small intestine tissue

Rat

Abstract

2Escherichia coli bacteria are often found in the human digestive system. The purpose of this research is to investigate the effect of Probiotic mixture (Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus heloticus) on the damage of the Small intestine tissue of male Rats infected with Escherichia coli bacteria. In this experimental study, 21 adult male Wistar Rats were divided into three groups of seven: control, infected with Escherichia coli bacteria (108 CFU/ml), and treated with Probiotic mixture. Escherichia coli and Probiotics (109 CFU/ml) were given to Rats by gavage method. At the end of the experiment, the Rats were euthanized, then tissue sections were prepared from the small intestine, the samples were stained by hematoxylin-eosin method and studied by light microscope. Small intestinal tissue damage showed a significant decrease in the Probiotic treatment group compared to the bacteria-infected group (P<0.05). According to the findings of this research, Probiotics can probably be used as a new application in a wide range of pharmaceutical products for the purposes of prevention or improvement of treatment methods.

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