OPTIMIZATION OF TISSUE CULTURE CONDITION IN LENTIL (LENS CULINARIS MEDIK. CV. GACHSARAN) TO INDUCE EFFECTIVE MULTIPLE SHOOT INDUCTION

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ZAKER TAVALLAIE F.; BAGHERI A.; GHAREHYAZI B.; SHARMA K.

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Paper Information

Journal: Iranian Journal of Field Crops Research Year:2009 | Volume:7 | Issue:2 Page(s): 411-419

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Title

OPTIMIZATION OF TISSUE CULTURE CONDITION IN LENTIL (LENS CULINARIS MEDIK. CV. GACHSARAN) TO INDUCE EFFECTIVE MULTIPLE SHOOT INDUCTION

Author(s)

ZAKER TAVALLAIE F. | BAGHERI A. | GHAREHYAZI B. | SHARMA K. | Issue Writer Certificate

Keywords

LENTIL (LENS CULINARIS M.)Q2

MULTIPLE SHOOT INDUCTIONQ2

HORMONESQ2

EXPLANTSQ2

Abstract

Optimization of regeneration in lentil is necessary to improve this plant by novel methods. First stage of regeneration is efficient MULTIPLE SHOOT INDUCTION using proper EXPLANTS. In this research, four EXPLANTS of leaflet, stem, cotyledon without embryo axes and cotyledon with embryo axes were tested to determine the best explant. Probably effect of BAP in germination media of seedling tested on shoot induction of EXPLANTS. There was comparison between effect of different level and combination of various cytokinins on MULTIPLE SHOOT INDUCTION. Effect of explant age and pH of media also tested on shoot induction. Results showed that, cotyledon with embryo axes is the best explant. Existence of BAP in germination media was very useful. There was highest shoot induction percentage (until to 96%) in MS media complemented with 7.5mM 2ip, 4mM Kin and 2 mM TDZ. Using this media produced more than 40 shoots per explant in some samples. Age of explant and pH of media was effective on MULTIPLE SHOOT INDUCTION. Results of primary research in gene transformation of lentil indicated efficient ability of this protocol to use in gene transformation.

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APA: Copy

ZAKER TAVALLAIE, F., BAGHERI, A., GHAREHYAZI, B., & SHARMA, K.. (2009). OPTIMIZATION OF TISSUE CULTURE CONDITION IN LENTIL (LENS CULINARIS MEDIK. CV. GACHSARAN) TO INDUCE EFFECTIVE MULTIPLE SHOOT INDUCTION. IRANIAN JOURNAL OF FIELD CROPS RESEARCH, 7(2), 411-419. SID. https://sid.ir/paper/119156/en

Vancouver: Copy

ZAKER TAVALLAIE F., BAGHERI A., GHAREHYAZI B., SHARMA K.. OPTIMIZATION OF TISSUE CULTURE CONDITION IN LENTIL (LENS CULINARIS MEDIK. CV. GACHSARAN) TO INDUCE EFFECTIVE MULTIPLE SHOOT INDUCTION. IRANIAN JOURNAL OF FIELD CROPS RESEARCH[Internet]. 2009;7(2):411-419. Available from: https://sid.ir/paper/119156/en

IEEE: Copy

F. ZAKER TAVALLAIE, A. BAGHERI, B. GHAREHYAZI, and K. SHARMA, “OPTIMIZATION OF TISSUE CULTURE CONDITION IN LENTIL (LENS CULINARIS MEDIK. CV. GACHSARAN) TO INDUCE EFFECTIVE MULTIPLE SHOOT INDUCTION,” IRANIAN JOURNAL OF FIELD CROPS RESEARCH, vol. 7, no. 2, pp. 411–419, 2009, [Online]. Available: https://sid.ir/paper/119156/en

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