MOLECULAR DETECTION OF PATHOGENIC LEPTOSPIRA IN IRAN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AGHAEIPOUR KH.; SAFAVIEH S.A.S.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARCHIVES OF RAZI INSTITUTE Year:2007 | Volume:62 | Issue:4 Page(s): 191-197

Download Full-Text

Persian Verion

View:

1,156

Download:

156

Cites:

Information Journal Paper

Title

MOLECULAR DETECTION OF PATHOGENIC LEPTOSPIRA IN IRAN

Author(s)

AGHAEIPOUR KH. | SAFAVIEH S.A.S. | Issue Writer Certificate

Keywords

LEPTOSPIRAQ4

PCRQ3

MOLECULAR DIAGNOSISQ4

MAT AND LEPTOSPIROSISQ4

Abstract

Leptospirosis is an acute infectious, systemic and septisemic disease which had recent outbreaks in some parts of Iran especially in north provinces. Rapid detection is a critical step for treatment and control ofthis disease. In this research a PCR based method was evaluated for detection of Iranian local endemic serovars. All reported endemic serovars of LEPTOSPIRA including LEPTOSPIRA grippotyphosa, LEPTOSPIRA canicola, LEPTOSPIRA sejreo harcijo, LEPTOSPIRA pomona and LEPTOSPIRA icterohaemorrhagiae which at present are used for LEPTOSPIRA micro agglutination test (LMAT) in Iran, were subjected to this PCR. Standard representative serovars from ATCC studied in parallel to local serovars. DNA extracted by phenolchloro form- isoamyl alcohol precipitation. After optimization, the sensitivity of PCR was about 1 fg equal to DNA of 1 LEPTOSPIRA. All studied serovars including non pathogenic L. biflexa produced the reported 285 bp fragment. Restriction analysis with MboI confirmed the PCR product accuracy. In case of L. biflexa the pattern was completely different from the pathogenic ones. None of near matching bacteria had product in this method. This system was able to detect the existence of LEPTOSPIRA DNA in all of studied LMAT positive serums.

Cites

DETECTION OF PATHOGENIC LEPTOSPIRA SPP. BY POLYMERASE CHAIN REACTION REACTION (PCR) TARGETING LIGB GENE

References

No record.

Cite

APA: Copy

AGHAEIPOUR, KH., & SAFAVIEH, S.A.S.. (2007). MOLECULAR DETECTION OF PATHOGENIC LEPTOSPIRA IN IRAN . ARCHIVES OF RAZI INSTITUTE, 62(4), 191-197. SID. https://sid.ir/paper/120027/en

Vancouver: Copy

AGHAEIPOUR KH., SAFAVIEH S.A.S.. MOLECULAR DETECTION OF PATHOGENIC LEPTOSPIRA IN IRAN . ARCHIVES OF RAZI INSTITUTE[Internet]. 2007;62(4):191-197. Available from: https://sid.ir/paper/120027/en

IEEE: Copy

KH. AGHAEIPOUR, and S.A.S. SAFAVIEH, “MOLECULAR DETECTION OF PATHOGENIC LEPTOSPIRA IN IRAN ,” ARCHIVES OF RAZI INSTITUTE, vol. 62, no. 4, pp. 191–197, 2007, [Online]. Available: https://sid.ir/paper/120027/en

Related Journal Papers