Laboratory diagnosis challenges of Covid-19: a Real-time RT-PCR assaybased survey

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Erfanian Saiedeh; javeshghani Danesh; FARHANG MOHSEN; ROUSTAZADEH ABAZAR

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Journal: Pars Journal of Medical Sciences Year:2021 | Volume:19 | Issue:1 Page(s): 63-68

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Title

Laboratory diagnosis challenges of Covid-19: a Real-time RT-PCR assaybased survey

Author(s)

Erfanian Saiedeh | javeshghani Danesh | FARHANG MOHSEN | ROUSTAZADEH ABAZAR | Issue Writer Certificate

Keywords

Coronavirus

Polymerase Chain Reaction

Molecular Study

Quality Control

Abstract

Introduction: A novel Coronavirus has recently been discovered in Wuhan, China in December 2019 named SARSCOV-2. Sensitive molecular assays are now available for detection of SARS-COV-2. The aims of this study were to detect positive subjects of SARS-COV-2 and to survey some challenges that are encountered practically in detection of the virus by Real-time RT-PCR (rRT-PCR) technique. Materials and Methods: 271 subjects including 105 women and 166 men were registered by census method from 15 April to 15 Jun of 2020. Nasopharyngeal and oropharyngeal swab samples of suspicious cases were collected for RNA extraction to identify SARS-COV-2 by rRT-PCR amplification. Results: 52 women and 82 men were positive for Covid-19. However, technically the results showed that in the time period of 15 April to 15 May of 2020 the contents of received rRT-PCR kits were infected with positive control. 8 % of certain negative control samples showed a peak resembling to that of the positive samples in the rRT-PCR process. In addition, the results showed that in some of the rRT-PCR kits the master mix would produce peaks like positive control without adding the sample to it automatically in 7 % of the tests. Conclusion: Having different types of negative control for diagnosis of SARS-COV-2 is necessary in extraction and rRT-PCR steps to reduce the chance of having false positive results.

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APA: Copy

Erfanian, Saiedeh, javeshghani, Danesh, FARHANG, MOHSEN, & ROUSTAZADEH, ABAZAR. (2021). Laboratory diagnosis challenges of Covid-19: a Real-time RT-PCR assaybased survey. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), 19(1 ), 63-68. SID. https://sid.ir/paper/954415/en

Vancouver: Copy

Erfanian Saiedeh, javeshghani Danesh, FARHANG MOHSEN, ROUSTAZADEH ABAZAR. Laboratory diagnosis challenges of Covid-19: a Real-time RT-PCR assaybased survey. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL)[Internet]. 2021;19(1 ):63-68. Available from: https://sid.ir/paper/954415/en

IEEE: Copy

Saiedeh Erfanian, Danesh javeshghani, MOHSEN FARHANG, and ABAZAR ROUSTAZADEH, “Laboratory diagnosis challenges of Covid-19: a Real-time RT-PCR assaybased survey,” PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), vol. 19, no. 1 , pp. 63–68, 2021, [Online]. Available: https://sid.ir/paper/954415/en

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